Spectroscopy and Dilution Flashcards
1
Q
What is a chromophore?
A
A chromophore is the part of a molecule which when exposed to visible light will absorb and reflect a certain colour
2
Q
What is blue dextran?
A
A large polysaccharide containing attached blue dye
3
Q
What is haemoglobin?
A
The major protein found in red blood cells
It contains an iron-haem group that binds oxygen
4
Q
What type of light do proteins absorb?
A
Proteins absorb light in the UV range
5
Q
What is the equation for absorbance using a spectrophotometer?
A
Absorbance = log (Incidence light/transmitted light)
6
Q
What does molarity mean?
A
Moles per litre
mol/L
7
Q
What is the equation for molarity?
A
(Grams/Litre)/(Molecular Mass)
Molarity = (g/L)/(g/mol)
8
Q
What is the dilution equation?
A
C1V1 = C2V2
C1 = stock conc V1 = stock vol C2 = dilution conc V2 = dilution volume
