the genome topic questions

Question 1

Explain why the enzyme protease was added to the mixture(2)

Answer 1

Protease breaks down proteins.(1)
In the cell membrane(1)
Destroy enzymes that may breakdown the DNA(1)

Question 2

DNA can be extracted from fruit
Describe how cells are broken down to release DNA(2)

Answer 2

Homogenise cells(1)
mix cells with salt/detergent(solution)(1)

Question 3

Describe the structure of DNA and how it can be extracted from the nucleus(6)

Answer 3

DNA structure
Double helix
weak hydrogen bonds
Polymer

DNA extraction
crush up
add detergent
heat in water bath
add it to cold ethanol
DNA forms as a perciptate white strands

Question 4

What is the role of ethanol in DNA extraction

Answer 4

to preciptate the DNA(1)

Question 5

Give a reason for filtering the mixture(1)

Answer 5

to remove insouluble material(1)

Question 6

State two other variables that the student should control when using this method(2)

Answer 6

mass of fruit(1)
Volume of ethanol(1)