the fundamental unit of life. Flashcards

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1
Q

write a short note on cells.

A

innumerable kinds of organisms live on our planet earth. all these organisms look different from each other and are broadly classified as bacteria, protists, fungi, plants and animals. however, all of them are made up of microscopic units, called cells. in some cases, single cell functions as an entire organism. such organisms are called unicellular organisms e.g, bacteria, amoeba, paramecium. in others, many cells group together and acquire different functions to form various body parts. such organisms are termed as multicellular organisms e.g some fungi, plants and animals. organisms have cells to perform various metabolic reactions in a delicately balanced environment to sustain life. cells, in living organisms act as life supporting chambers and provide them such a special balanced environment. in other words, every living cell keeps its chemical composition steady within its boundary and, thus, is capable of performing the activities in a desired manner. cell is regarded as the structural and functional unit of living organisms.

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2
Q

how was the cell discovered?

A

the term cell was introduced by an english scientist, robert hooke in his book micrographia, published in the year 1665. he examined thin slices of cork under his self designed primitive microscope. the cork is a substance obtained from the bark of a spanish oak tree, Quercus. he was suprised to see that the cork resembled the structure of a honey comb and consisted of many small compartments. in fact, he saw the dead cells of plants ( only cell walls which looked like empty rooms. these dead cells had lost their living contents. he called these rooms as cellulae, now termed as cells. cellulae is a latin word which means a little room.
this simple observation which seems to be a very small and insignificant, was an extremely important discovery in the history of biological sciences. it was the first observation which showed that organisms were made up of small units, called cells. robert hooke subsequently used his primitive microscope to observe many other plant cells.

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3
Q

explain the history of cell in detail.

A
  1. robert hooke coined the tern cell in 1665.
  2. anton von leeunwenheok ( 1632-1723 ) designed improved microscope and described, for the first time, free living cells in 1674. later, he also described protozoans, red blood corpuscles and human sperm cells.
    ( with the advent of more sophisticated tools and techniques, cell study developed rapidly in the nineteenth century )
  3. in 1831, a scotch botanist robert brown ( 1773-1858 ) discovered the nucleus in an orchid root cell. By this time, it had also been found that the cells were surrounded by some sort of limiting structure, the cell membrane.
  4. a French zoologist dujardin, discovered in 1835 a semi fluid living material in certain protozoans and he named it as sarcode.
  5. in 1840, __ johannes E. purkinje noted similar material in plant cells and named it protoplasm, the first substance.
  6. a German botanist M.J schleiden, in 1838, announced that all plants were composed of cells. a year later, german zoologist, T. schwann stated that all animals are also formed of cells. schwann found that, except for the cell wall, the animal cells more or less resembled the plant cells in having a nucleus located in a clear substance bounded by a cell membrane. These findings formed the basis of cell theory.
  7. in 1858, another German biologist rudolph virchow ( 1821- 1920 ) presented the idea omnis cellula e cellula which means that all living cells arise from the pre existing cells. this led to the modification of cell theory.
  8. haeckal, in 1866 reported that nucleus stores and transmits hereditary information.
  9. electron microscope was discovered by knoll and ruska in 1940. Discovery of electron microscope made it possible for us to observe and understand the complex structure of the cell and its various cell organelles, i.e, it’s ultrastructure
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4
Q

why is the term cell considered a misnomer?

A

We now know that the really important parts of the cell are its contents and the cell may or may not have a cell wall. thus, the term cell is a misnomer. misnomer is a wrong or inaccurate name. The term cell is considered a misnomer because the literal meaning of cell is a hollow space or chamber. however, a cell is not hollow. the word cell is still used to describe the structural and functional unit of life.

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5
Q

what is the instrument that is used for studying cells?

A

Cells are microscopic in structure and cannot be seen with a naked eye. They are studied with the help of instruments, called microscopes. These provide enlarged images of cells and the structures they contain.
microscope is the most common optical instrument used to observe cellular organisation of living organisms.
there are different kids of microscopes which can be broadly divided into two categories:
1. light microscopes.
2. electron microscopes.

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6
Q

what is a simple microscope?

A

Simple microscopes are just magnifying glasses. In fact, human eye works as an optical instrument and the eye lens acts as a simple lens. The resolving power of a healthy young human eye is approximately 0-1mm at 25cm viewing distance. any object smaller than this cannot be viewed by the naked eyes. a microscope is, therefore, used for enlargement and magnifying the smaller object so that it becomes visible by the human eye.

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7
Q

what is a compound microscope? explain in detail.

A

a compound microscope is one in which magnification takes place is two stages. it consists of two lens units __ the first, called the objective, produces a primary magnified image. and the second lens unit, called eye piece or ocular magnifies the first image. such microscopes employing two lens units in working position at one time are called monocular monoobjective microscopes.
The simple student’s microscope, generally used in the biological laboratory to observe tiny cells is called compound microscope. Such a microscope uses light, generally sunlight to illuminate the object or specimen under study, and hence it is also called light microscope. it has a magnification of 100-150. to magnify the object, power of objective ( 10x, 40x, 100x ) and eye piece ( 10x, 15x ) is used.
The light or compound microscope is a **strong, heavy metal instrument which comprises of u shaped base having two vertical pillars. a curved arm is movably joined to the pillars to hold the microscope. The arm can be bent over the pillars at inclination joint to suit the viewer. The upper part of the arm holds the movable body tube. The other parts of the microscope are reflector, condenser lens, stage, objective lens, ocular lens ( eye piece ), and adjustment screws ( coarse and fine ).
a glass slide, containing the object or specimen under study, is kept on a stage under an objective lens. Light is then passed through the object or the specimen with the help of a mirror ( reflector ) and the condenser from below the stage. A magnified, sharp image of the object/specimen can thus be seen through the ocular lens/eye piece by focusing the coarse and fine adjustment knobs properly. We can increase or decrease the magnification of the image by changing the objective lens of high or low power.

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8
Q

what is a electron microscope?

A

it is a very large instrument that helps in observing sub-cellular structures which cannot be seen with a light microscope. it uses very high voltage electricity. For its working, internal vacuum is essential. It uses electromagnets instead of glass lenses and beam of electrons instead of light. Ultra thin and dry section of material ( under study ) is impregnated with some metal to enhance the contrast and the image is obtained on a photographic plate or fluorescent screen. Magnification is 1,00,000 to 5,00,000 times.

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9
Q

what are unicellular organisms?

A

organisms wherein a single cell constitutes the whole organism. *amoeba, paramecium, chlamydomonas ( all protists ), bacteria ( prokaryotes ) and yeasts ( fungi ) are few examples of such organisms. these single celled organisms live independently in nature and their single cells are capable of performing all the life processes such as obtaining food, respiration, metabolism, excretion, growth and reproduction

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10
Q

what are multicellular organisms?

A

These organism are formed of many cells. in fact, number of cells group together and acquire different functions to form various body parts in multicellular organisms. Examples are fungi, plants and animals including human beings. In fact, the multi cellular organism has come into existence from a single cell. for example, fertilization of eggs with sperm results in the formation of a single celled zygote. This single cell divides and redivides in various planes to produce a multicellular body. The cells further divide to produce different organs of a multicellular organism. thus, all cells arise from pre existing cells and this confirms the theory of cell lineage proposed by virchow ( 1858 ). according to the theory, all cells arise from pre existing cells.
All cells, whether they exist as independent unicellular organisms or as part of a multicellular organism, have certain structures in common to carry out basic functions essential for the survival of the cells. Every living cell has the capacity to perform certain basic functions that are characteristic of all living organisms.
how does a living cell perform these basic functions?
All multicellular organisms show division of labour. it means that different parts of a multicellular organism perform different functions. Each cell possesses specific components within it known as cell organelles. Each kind of cell organelle performs a special function. For instance:
1. Making of new material in the cell such as Protein synthesis by ribosomes and food synthesis ( carbohydrate ) by chloroplasts.
2. Energy generation in the form of ATP ( adenosine triphosphate ) by mitochondria.
3. Clearing up the waste substances from the cell by lysosomes
these organelles together constitute the basic building block called the cell and the cell is able to live and perform its functions because of these organelles.

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11
Q

Differentiate between unicellular and multicellular organisms.

A

UNICELLULAR ORGANISMS:
1. consists of a single cell.
2. the single cells performs all the life activities.
3. there is no division of labour as the single cell performs all the activities.
4. single cell is involved in the production of new organisms during reproduction.

MULTICELLULAR ORGANISMS:
1. It consists of a large number of cells
2. a single cell performs one or a few activities
3. There is division of labour as cells are _ specialized_ to perform different functions of the body
4. only germ cells take part in reproduction.

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12
Q

Write a short note on the shape, size and number of cells.

A

cells vary in shape, size and number. Unicellular organisms are identified on the basis of their shape. However, some unicellular organisms, e.g, amoeba, are irregular in shape. In multicellular organisms, the shape of cells depends upon their position in the body, specific function which they perform and their interaction with neighbouring cells.
Cells also vary in size. Their dimensions are usually expressed in microns or micrometers ( um ) and angstrom ( Å ). Most human cells typically range in diameter from 8-25 um. They are observed under the microscope. The small objects which can be seen only with the help of microscope are called microscopic in size. However, there are some cells which are either extremely small or extremely large in size.

         1um = 10^-3mm or 1/1000nm
        1nm = 10^-3um or 10^-6mm
       1Å = 10^-1nm or 10^-7mm or 10^-10m
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13
Q

what are prokaryotic and eukaryotic cells?

A

Prokaryotic cell
The cells of some most primitive organisms such as bacteria and blue green, i.e, cyanobacteria lack nuclear membrane around their genetic materials and hence are called prokaryotic cells or prokaryotes ( pro_before, karyon_nucleus ). The genetic material in these cells lies in direct contact with the cytoplasm and is called nucleod. prokaryotes also do not contain membrane bound organelles in the cytoplasm. ribosomes are, however, present.

eukaryotic cell
The cells of other organisms possess true nucleus bounded by nuclear membrane. suck organisms are called eukaryotes. The eukaryotes may be unicellular or multicellular organisms. Examples include protists, fungi, plants and animals. Every Eukaryotic cell contains a plasma membrane, membrane bound nucleus containing the genetic material and other membrane bound subcellular organelles in the cytoplasm. The organelles divide the cytoplasm into compartments to faciliate specific metabolic functions

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14
Q

give the detailed structure of a eukaryotic cell.

A

no cell is completely unspecialized so as to be considered a typical cell. most of the cells have certain common subcellular structure. a eukaryotic cell has three main components:
1. plasma membrane/cell membrane.
every cell is enclosed on all sides by a distinct covering, called plasma membrane or cell membrane. it is living, ultra thin structure made of lipid and proteins. it keeps the cell contents seperated seperated from the external environment. the plasma membrane allows/permits the entry and exit of only selected materials and hence, is called a selectively permeable membrane.
2. nucleus.
Each eukaryotic cell has a deeply stained rounded structure, called nucleus. It is located centrally in animal cells but pushed to one side due to large vacuole in plant cells. It is the most conspicuous and the largest organelle of an Eukaryotic cell. Nucleus contains genetic material.
3. cytoplasm.
The fluid and semi fluid matrix filled in between the nucleus and the plasma membrane is called the cytoplasm. It contains various specialized cell organelles. Each cell organelle performs specific function for the cell. All the components of a cell taken together keep it living. No part can survive is separated from the cell.

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15
Q

Explain plasma membrane.

A

each cell, prokaryotic as well as eukaryotic, is surrounded by a covering called the plasma membrane or plasmalemma or cell membrane. Also, most cell organelles in Eukaryotic cell ( e.g mitochondria, plastids, golgi apparatus, lysosomes, endoplasmic reticulum, peroxisomes, vacuoles etc. ) are enclosed by subcellular unit membranes. These membranes, thus, compartmentalise the cell. however, neither the cell nor the compartments in it are totally isolated from the surrounding medium. The membrane, in fact, allow continuous flow of selected materials across them as required from time to time.

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16
Q

what are the different kinds of permeability?

A

1. impermeable. if a membrane does not allow both solvent and solute particles to pass through it, it is called impermeable membrane, e.g, cuticle layer.
2. permeable. if a membrane allows both the solvent and solute particles to pass through it freely, the membrane is called permeable, e.g, primary cell wall.
3. semi permeable. if a membrane allows penetration of only solvent molecules but not the solute particles, it is called semi permeable, e.g, artificial vapour membrane.
4. selectively permeable. if a membrane allows penetration of solvent freely but selects the passage of specific solute particles, it is called selectively permeable membrane, e.g plasma membrane

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17
Q

explain the molecular structure of cell membrane

A

Plasma membrane is a living, ultra thin, elastic, selectively permeable membrane that appears as a mere line under the light microscope. However, its detailed molecular structure can be studied under an electron microscope. Basically all the plasma membrane are composed of proteins, lipids and small fractions of carbohydrates. fine structure of plasma membrane, as revealed by electron microscopic studies, appeared to be three layered ( trilaminar membrane ).

lamellar model
this model was proposed by danielli and davson in 1935 and J. D. robertson in 1959 to explain the structural organization of plasma membrane. according to these scientists, the plasma membrane has three layers ( dark-light dark ). A central light lipid bilayer is sandwitched between two protein monolayers ( P-L-L-P )
fluid mosaic model
This model is the most recent and accepted model proposed by singer and nicolson in 1972. according to this model, the cell membrane is made up of a lipid bilayer and two types of protein molecules. the lipid bilayer forms a highly viscous liquid in which the two types of protein molecules ( intrinsic proteins and extrinsic proteins ) are organized in a mosaic manner. intrinsic proteins are embedded in the lipid bilayer incompletely or completely, and the extrinsic proteins occur superficially.
Fluid mosaic model provides satisfactory explanation of the structure and functions of plasma membrane

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18
Q

what are the function of cell membrane/plasma membrane?

A
  1. it gives form to the cell. (shape )
  2. it maintains individuality of the cell.
  3. it keeps the cell contents in place and prevent mixing with the extracellular materials.
  4. protects the cell from injury.
  5. it is selectively permeable i.e it regulates the flow of selected materials into an out of the cell.
  6. it forms organelles with the cytoplasm.
  7. its junctions ( point where two or more things are joined. ) keep the cells together.
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19
Q

explain the transport of materials across cell membrane

A

in order to perform various life activities, all cells must take up and turn out materials through the cell membrane. The plasma membranes acts as physical barriers between the cell and its surrounding environment and between cell organelles and the surrounding cytoplasm.
the cell membranes are not freely permeable, i.e., they do not allow movement of all kinds of substances across the. instead, they are selectively permeable, i.e., they allow the entry or exit of only selected materials. thus, selective permeability of plasma membrane enables the cell to maintain homeostasis, i.e., a constant internal environment despite the changes outside it. cell membranes allow the moment of different materials across them differently.

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20
Q

what are the advantages of selective permeability?

A

selective permeability of plasma membrane ensures that:
1. useful molecules enter the cell.
2. metabolic intermediates remain within the cell.
3. secretions and waste leave the cell.

. . the substances generally drawn in the cell include:
__ raw materials for metabolism, viz., food stuffs, water, salts and oxygen.
__ regulatory substances, e.g, vitamins and hormones.

. . the substances generally drawn out of the cell include:
__ the products of metabolism, namely, nitrogenous wastes and carbon dioxide.
__ secretions.

21
Q

what are the mechanisms involved in the entry or exit of various materials?

A
  1. passive transport.
  2. active transport.
  3. bulk transport.
22
Q

explain the passive transport of materials. ( cell membrane )

A

passive transport of materials is slow and does not expend energy, it occurs down the concentration gradient and does not use carrier proteins. only small molecules ( e.g., water, gases and ions. ) pass through the plasma membrane by passive transport. it is of two types,
1. diffusion.
2. osmosis.

23
Q

explain the diffusion of materials in passive transport.

A

very small sized substances such as molecules and ions, water, gases ( e.g., oxygen, carbon dioxide ) etc. generally move across the plasma membrane by a process called diffusion. the process by which a substance uniformly spreads into another substance by random movement of its particles from a region of higher concentration to a region of its lower concentration due to their kinetic energy is called diffusion.
it is faster in gaseous phase than in liquid phase or solid phase.

24
Q

demonstrate diffusion by an activity and relate it with our body cells?

A

take a glass beaker or a glass tumbler more than half filled with clear water. now put few crystals of copper sulphate or few drops of ink in water and observe. you could find that crystals of copper sulphate or ink molecules diffuse into water slowly until these molecules get uniformly spread in water turning it into a coloured solution.
similar phenomena, as depicted in the above activity, occur in out body cells. for instance, carbon dioxide is produced as an end product of metabolism in cells and its concentration inside the cell becomes high. however, the concentration of carbon dioxide in the external environment of cells is low. Due to difference in the concentration of carbon dioxide between the inside and outside of the cells, carbon dioxide moves out from the region of its higher concentration to the region of its low concentration through plasma membrane of respective cells by diffusion. This process goes on till the concentration of carbon dioxide on both the sides becomes equal. Similarly, oxygen enters into the cells by diffusion, i.e., from region of its higher concentration outside the cells to region of its lower concentration inside the cells. In the same way, large number of small molecules keep on moving inside the cells or out of the cells by diffusion in our body.

25
Q

what is the significance of diffusion?

A
  1. it helps in the distribution of various substances throughout the cytoplasm of the cell without much delay.
  2. it helps in the exchange of respiratory gases ( oxygen and carbon dioxide ) between the body cells and their environment.
  3. various materials such as gases, liquids and solids dissolve in the medium, i.e., air or liquid by diffusion.
  4. loss of water in vapours form from the aerial parts of the plants ( transpiration ) occurs through diffusion.
  5. flowers of plants spread aroma through diffusion. it attracts insects and other plants for pollination.
26
Q

explain the osmosis of materials in passive transport.

A

if two solutions having different concentration of a solute are seperated by a semipermeable or selectively permeable membrane, i.e., a membrane which is permeable to the small molecules, e.g., water molecules and not permeable to large molecules of dissolved substances, e.g., sugar molecules, then a different type of diffusion occurs. in such a case, one would observe movement of solvent from the region of its higher concentration to the region of its lower concentration across the semipermeable. it can also be interpreted as movement of solvent fom the region of lower concentration of solute to the region of its higher concentration through semipermeable membrane. such a phenomenon is called osmosis.
the diffusion of water or solvent through a semipermeable membrane from a solution of lower concentration of solutes to a solution of higher concentration of solutes to which the membrane is selectively impermeable, is called osmosis.

27
Q

what are the types of osmosis?

A

the two types of osmosis:
1. endosmosis.
2. exosmosis.

28
Q

what is endosmosis?

A

It is the entry of water molecules into the cells through semipermeable plasma membrane when surrounded by hypotonic solution.

29
Q

what is exosmosis?

A

It is the exit of water molecules from the cells through semi permeable cell membrane when surrounded by hypertonic solution.

30
Q

write down an experiment to demonstrate osmosis in the laboratory.

A

requirements: funnel fitted with a semi permeable membrane, beaker, sugar solution, water.
procedure: take sugar solution in a funnel fitted with a semipermeable membrane ( fish bladder or egg membrane ) upto mark A and place it into an inverted position in a beaker filled with clean water. after some time, observe the level of sugar solution in the funnel.
result: you would find that the sugar solution has risen from level A to level B.
explanation &. conclusion: sugar solution in the funnel and water in the beaker are seperated by a semipermeable membrane. the fitted membrane is permeable to small water molecules but is relatively impermeable to large sugar molecules dissolved in water. due to difference in the concentration of solute on the two sides of semipermeable membrane, water molecules have moved from the solution having lower concentration of solutes ( e.g, water ) to the solution having higher concentration of solutes ( e.g, sugar solution ) due to osmosis. as a result, sugar solution has risen to new level B.

31
Q

We can use three different concentrations of salt or glucose solutions with reference to solute concentration in red blood cells. what are thes solutions?

A

these solutions are termed as:
1. isotonic solution: isotonic solution is one in which the concentration of water and solutes is the same as in the cytoplasm of the red blood cells. 0.9% salt solution and 5% glucose solution are isotonic for red blood cells.
2. hypotonic solution: hypotonic solution is one in which the concentration of solutes is less and concentration of water is more as compared to inside the red blood cells. 0.66% salt solution and 0.2% glucose solution are hypotonic for red blood cells.
3. hypertonic solution: hypertonic solution is one in which the concentration of solute is more and the concentration of water is less as compared to in the cytoplasm of the red blood cells. 1.25% salt solution and 10% glucose solution are hypertonic for red blood cells.

32
Q

write down an experiment to demonstrate with human red blood cells.

A

requirement: glass slides, disposable needle, ringer’s solution, water, concentrated salt solution ( hypertonic solution ), cotton, rectified spirit.
procedure: clean the ring finger of your left hand with rectified spirit and then prick it with a disposable needle. press the finger slightly and take one drop each on the three clean glass slides marked A, B, C. now, add
1. a drop of ringer’s solution ( isotonic solution ) on drop of blood on slide A,
2. a drop of water ( hypotonic solution ) on drop of blood on slide B,
3. a drop of concentrated salt solution ( hypertonic solution ) on drop of blood on slide C.
*observe all the three slides under the light microscope one by one.**
observations: 1. on slide A, you will notice normal circular, biconcave, non-nucleated red blood cell
2. on slide B, you will observe swollen or bursted ( haemolysed ) red blood cells.
3. on slide C, you will find *shrunken ( crenated ) red blood cells.
explanation and solutions: 1. on slide A, red blood cells are placed in isotonic Ringer’s solution, which has the same concentration of water and solutes as is present in the cytoplasm of the red blood cells. therefore, the small water molecules leave or enter the red blood cells through semipermeable plasma membranes at the same rate and there is no net movement of water across the plasma membranes of the red blood cells. therefore, the red blood cells appear normal circular, biconcave and non-nucleated.
2. on slide B, red blood cells are placed in water ( hypotonic solution ) which has lesser concentration of solutes and greater concentration of water than in th cytoplasm of the red blood cells. therefore, due to osmosis, more water molecules go inside the red blood cells from the surrounding hypotonic medium through semipermeable plasma membranes in an attempt to equalize the water concentration on both the sides of the membranes. consequently, red blood cells swell up and burst ( haemolysed )
3. on slide C, red blood cells are placed in hypertonic solution ( concentrated salt solution ) which has greater salt concentration and less water concentration than in the cytoplasm of the red blood cells. therefore, red blood cells lose more water into the surrounding medium in an attempt to equalize water concentration on both the sides of the plasma membrane. consequently, they shrink and appear wrinkled ( crenated ).

33
Q

give some more examples of osmosis.

A
  1. fresh water unicellular organisms ( e.g, amoeba, paramecium ) continuosly gain water in their bodies due to osmosis. these organisms have mechanisms ( e.g contractile vacuoles ) to throw out excess of water from their bodies.
  2. most plant cells have the tendency to gain water due to osmosis.
  3. absorption of water by the plant roots from the soil through root hairs is also an example of osmosis.
  4. certain plant movements ( e.g, seismonastic movements in touch me not plant ) occur due to loss or gain of water.
  5. stomata are present in the leaves. they open and close at different time of the day due to osmotic movements of water.
  6. in plants, cells, tissues and soft organs ( leaves, young shoots, flowers ) maintain turgidity or stretched form due to osmotic absorption of water.
34
Q

what is the difference between diffusion and osmosis?

A

diffusion:
1. diffusion can occur both in air and liquid ( water ) medium.
2. it involvesmovement of molecules ( solids, liquids or gases ) from the region of their higher concentration to the region of their lower concentration.
3. it can occur without of through a semipermeable membrane.
4. it equalizes the concentration of diffusable molecules throughout the medium.
5. it is dependent upon the kinetic energy of the molecules of diffusing substance only.

osmosis:
1. osmosis occurs only in liquid medium.
2. it involves movement of solvent molecules only from the region of their higher concentration to the region of their lower concentration.
3. it always takes place through a semipermeable membrane.
4. it does not equalize the concentration of solvent molecules in the medium involved.
5. though it is the diffusion of solvent molecules only, yet it is influenced by the presence of solutes in the system.

35
Q

explain the active transport of materials.

A

this type of transport across plasma membrane is rapid and requires the use of energy in the form of ATP. it usually occurs against the concentration gradient and involves the use of carier proteins. glucose, amino acids and some ions ( e.g, Na+ and K+ ) pass through the plasma membrane by active transport or cotransport.

36
Q

what is the difference between active transport and diffusion?

A

active transport:
1. it is a rapid process.
2. it can move materials through a biomembrane against the concentration gradient.
3. it takes place in one direction only.
4. it needs carrier proteins to occur.
5. it uses energy of ATP.
6. it brings about selective uptake of materials.
7. it leads to accumulation of materials in the cell.

diffusion:
1. it is a slow process.
2. it can move materials through a biomembrane down the concentration gradient.
3. it takes place in both direction.
4. it occurs without carrier proteins
5. it does not use energy.
6. it it allows all transmissible molecules to pass through membranes.
7. it does not accumulation of materials in the cell.

37
Q

explain the bulk transport of materials.

A

it involves transport of large amounts of substances ( macromolecules, lipid droplets and solid food particles ) across the plasma membrane by utilizing energy. special processes are involved in the transport of such large quantities of materials. these include:
1. endocytosis. ( pinocytosis & phagocytosis ).
2. exocytosis.

38
Q

explain the process of endocytosis

A

endocytosis is the process by which animals engulf food and other substances from external medium by plasma membrane. the term endocytosis refers to invagination of a small region of plasma membrane, and ultimately forming an intracellular membrane bound vesicle. for instance, a unicellular amoeba acquires its food through this process.
depending upon the intake of fluid droplet or solid particles, endocytosis is of two types:
1. pinocytosis.
2. phagocytosis.

39
Q

explain pinocytosis.

A

It is the non specific intake of a tiny droplet of extracellular fluid by a cell through the cell membrane which cannot otherwise pass through it. It is also termed as cell drinking. It was first observed in amoeba. in this process, a small region of plasma membrane invaginates and the fluid droplet passes into the pocket so formed. This pocket is called caveola. the pocket depens and finally nips off as a fluid filled vacuole called pinosome or pinocytonic vesicle. thus, any material dissolved or suspended in the extracellular cell gets into the cell. the pinosome then shifts into the interior of the cell. pinocytosis may be important in the movement of the substances ( e.g, hormones or growth factors, some vitamins ) from one side of a cell to the other

40
Q

is endocytosis shown by both animals and plant cells? why/why not?

A

Only animal cells show endocytosis where plasma membrane is in direct contact with external medium. Endocytosis is not shown by plant cells because of their rigid cell wall and internal turgor pressure.

41
Q

explain phagocytosis.

A

phagocytosis is the intake of solid particles by a cell through cell membrane. it is also called cell eating. phagocytosis is the major feeding method in many unicellular organisms ( e.g, amoeba ) and simple metazoa ( e.g., sponges ). it is also the means by which leucocytes of blood engulf uninvited microbes ( e.g, viruses, bacteria ), cellular debris etc. in the blood. such cells are called phagocytes.
an area of the plasma membrane comes in contact with the food particles. the contact induces the cell membrane to put out tiny protoplasmic processes, the pseudopodia, around the food particles. the pseudopodia meet on the other side of the food particles and fuse. in this way, **an internal vacuole, called phagosome, containing food particles in a droplet of water is acquired.
thus, both pinocytosis and phagocytosis processes are imp. to:
1. bring materials into the cell by invagination and subsequently formation of a vesicle.
2. carry segments of cell membrane ( i.e, membrane of the vesicle ) into the cytoplasm.

42
Q

what is exocytosis?

A

exocytosis is the process that involves fusion of membrane of the exocytic vesicle with the plasma membrane to extrude its contents to the surrounding medium.
this process is also called cellular vomiting or ephagy and the vesicles that turn out the materials are termed exocytotic vesicles.
exocytosis process is responsible for:
1. removal of undigested food left in the food vacuoles in the cells.
2. secretion of substances such as hormones, enzymes.
3. replacement of internalized membrane by the fusion of exocytotic vesicles with the cell membrane.

43
Q

what is the difference between pinocytosis and phagocytosis?

A

pinocytosis:
1. it is the **intake of extracellular fluid droplets.
2. cell membrane invaginates to take up the material.
3. microfilaments play no role in endocytosis
4. it is a nutritive process
5. pinocytotic vesicles are only 0-1 micrometre.

phagocytosis:
1. it is the intake of extracellular particles.
2. cell membrane grows around the particle as pseudopodia.
3. microfilaments play an important role in phagocytosis.
4. it is a nutritive &. defensive process.
5. phagocytotic vesicles are 1 to 2 micrometre or more wide.

44
Q

what is cell wall?

A

the plant cells as well as cells or bacteria and fungi possess an additional layer outside the plasma membrane, called the cell wall. it is non living, rigid, completely permeable and is secreted by the cell itself to protect its contents. plant cell wall was first observed by robert hooke in 1665 in a thin slice of cork undr his primitive microscope.

45
Q

what is the physical structure of the cell wall.

A

young growing cells, meristematic cells, photosynthesizing cells, some storage cells, all parenchymatous cells and some other plant cells possess only a primary cell wall made up of cellulose. the primary cell walls of adjacent cells are cemented through middle lamella. however, many mature plant cells especially dead cells of xylem, cork and schlerenchyma possess an additional secondary wall ( in some cases tertiary wall also ) inner to the primary wall. The cell wall has narrow pits or pores through which fine cytoplasmic strands, called plasmodesmata , pass. the plasmodesmata form intercellular connections that allow exchange of materials between adjacent living cell contents.

46
Q

what is the chemical composition of cell wall?

A

the primary cell wall is chiefly composed of insoluble polysaccharide, cellulose ( a carbohydrate present in plant cells ). a cellulose molecule is a long unbranched chain of thousands of glucose units. the primary cell wall provides mechanical strength and protection to the protoplast. it is porous and considered permeable membrane. the secondary wall, deposited on the inner face of the primary wall, is composed of lignin and suberin substances. due to presence of lignin and suberin, the secondary wall becomes impermeable to the substances and the cell becomes dead.
middle lamella is chiefly of calcium and magnesium pectate. plant cell is living when it has only primary cell becomes dead when secondary wall is deposited on the inner face of the primary wall. thus, plant cells ( having only primary cell walls ) are able to absorb water by osmosis.

47
Q

what are the functions of plant cell?

A
  1. it maintains the shape of the cell.
  2. it protects the cells from **mechanical injury and prevents their dessication.
  3. it provides mechanical support against gravity. it is due to the rigid cell walls that the aerial parts of the plants are able to keep erect and expose their leaves to sunlight.
  4. being freely permeable, it allows the materials to **pass in and out of the cells.
  5. the plasmodesmata form intercellular connections that allow exchange of materials between adjacent living cell contents.
48
Q

explain plasmolysis.

A

A plant cell place in a hypotonic solution receives water by osmosis. It does not burst because it is surrounded by a rigid cell wall which can withstand the hydrostatic or turgor pressure of the turgid ( distended ) cell contents. The cell wall counters the turgor pressure by exerting wall pressure. This pressure stops the gain of water by a plant cell beyond a certain limit, i.e, when the cell contains become completely turgid.
If a plant cell is placed in hypertonic solution, the cytoplasm along with the plasma membrane shrinks and separates from the cell wall as water flows out from the vacuole of the cell. The vacuole shrinks but remains enclosed by the cytoplasm. This process of shrinkage of protoplast from the cell wall due to exosmosis caused by a hypertonic solution is called plasmolysis.
the phenomenon of shrinkage of protoplast from the cell wall due to exosmosis in a plant cell when placed in a hypertonic solution is called plasmolysis.

49
Q

what are the stages of plasmolysis?

A
  1. gradual loss of water from the plant cell causes the protoplast to stop exerting pressure on the cell wall. This is the beginning of plasmolysis. It is called limiting plasmolysis.
  2. Plasmolysis starts at the corners of the cell wall. As water flows out from the vacuole, the cytoplasm along with plasma membrane strinks and starts separating from the cell wall from the corners. This stage of plasmolysis is called incipient plasmolysis.
  3. Continuous loss of water from the vacuole of Plant Cell results in maximum shrinking of the protoplast. As a result, the cytoplasm along with plasma membrane loses contact from most the cell wall and comes to lie on one side of the cell wall. This stage of plasmolysis is called evident plasmolysis