The Final Exam 😨 Flashcards
Inductive Reasoning
Generalizations from specific observations
Deductive Reasoning
Specific conclusions from general principles
Evolution
Change in heritable characteristics over generations
Water Polarity
Oxygen slight negative charge, hydrogen slight positive charges
Natural Selection
Favorable traits lead to survival and reproduction
Acidic pH
pH below 7
Macromolecules
Carbohydrates, lipids, proteins, nucleic acids
Hydrolysis Reaction
Breaking molecules by adding a water molecule
Buffer
Solution resisting pH changes by absorbing/releasing ions
Dehydration Synthesis
Joining molecules by removing a water molecule
Disaccharide
Carbohydrate of two monosaccharide units
Monosaccharide
Single sugar molecule like glucose or fructose
Polysaccharide
Complex carbohydrate of many monosaccharide units
Purine Bases
Adenine and guanine nitrogenous bases
Amino Acid
Molecule with amino group, carboxyl group, and side chain
Pyrimidine Bases
Cytosine, thymine, uracil nitrogenous bases
ATP
Adenosine triphosphate, primary energy carrier in cells
DNA Base Pairing
Adenine with thymine, cytosine with guanine
Peptide Bond
Covalent bond joining amino acids in a protein
Protein Structure Levels
Primary, secondary, tertiary, quaternary
Enzyme
Biological catalyst speeding up chemical reactions
Denaturation
Loss of protein structure and function due to external factors
Saturated Fatty Acid
Single bonds between carbon atoms, saturated with hydrogen
Unsaturated Fatty Acid
Double bonds between carbon atoms, fewer hydrogen atoms
Triglyceride
Lipid with three fatty acid molecules bonded to glycerol
Steroid
Lipid molecule with a fused ring structure
Phospholipid
Lipid with hydrophilic head and hydrophobic tails
Robert Hooke
Discovered cells in cork slices, termed them ‘cells.’
Cell Theory
States all organisms are made of cells, the cell is the basic unit, and cells come from pre-existing cells.
Surface Area-to-Volume Ratio
High ratio in small cells aids in efficient exchange.
Light Microscope
Used for low magnification observations of cells.
Peptidoglycan
Structural component of bacterial cell walls.
Prokaryotes
Lack nucleus and organelles; include bacteria.
Archaea
Found in extreme environments like hot springs.
Rough Endoplasmic Reticulum
Site for protein synthesis and modification.
Endosymbiotic Theory
Mitochondria and chloroplasts originated as prokaryotes.
Virus Structure
Genome surrounded by protein capsid; may have envelope.
Bacteriophage Life Cycle
Attachment, injection, replication, assembly, and lysis.
Cell Membrane Composition
Made of phospholipids, proteins, cholesterol, and carbohydrates.
Phospholipid Bilayer
Hydrophilic heads outward, hydrophobic tails inward.
Integral Proteins
Embedded in membrane, can span its width.
Cholesterol in Membrane
Stabilizes fluidity, modulates membrane fluidity.
Passive Transport
Movement across membrane without energy input.
Osmosis
Diffusion of water across a selectively permeable membrane.
Simple Diffusion
Molecules move directly through lipid bilayer.
Endocytosis
Cell engulfs substances by forming vesicles.
Hypertonic Solution
Higher solute concentration outside cell, causing water loss.
First Law of Thermodynamics
Energy conservation law.
Redox Reactions
Involve electron transfer; reduction and oxidation.
Entropy
Measure of disorder in a system.
Second Law of Thermodynamics
Entropy tends to increase over time in closed systems.
ΔG (Gibbs Free Energy)
Determines spontaneity of a reaction.
Endergonic reactions
Require energy input, have positive ΔG, and are non-spontaneous.
ΔG
Gibbs free energy change; positive = non-spontaneous, negative = spontaneous.
Exergonic reactions
Release energy, have negative ΔG, and are spontaneous.
Activation energy
Energy needed to start a chemical reaction and overcome the barrier.
Catalyst
Speeds up reactions by lowering activation energy; like enzymes.
Substrate
Molecule acted upon by an enzyme in a chemical reaction.
Autotrophs
Organisms producing organic molecules from inorganic sources.
Heterotrophs
Organisms obtaining organic molecules from other organisms.
Stomata
Pores on leaves regulating gas exchange during photosynthesis.
Photosystem
Complex capturing light energy during photosynthesis.
Mitosis
Cell division for growth and repair, ensuring genetic fidelity.
Chloroplasts
Cell organelles where photosynthesis occurs.
Binary fission
Asexual reproduction in prokaryotes; cell division into two.
Kinetochore
Protein structure at the centromere for spindle fiber attachment.
Double-stranded DNA
Molecule carrying genetic information in cells.
Chromatin
DNA and histone protein complex forming chromosomes.
Homologous chromosomes
Chromosome pairs with the same genes but may have different alleles.
Sister chromatids
Identical chromosome copies produced by DNA replication.
Centromere
Region of a chromosome where sister chromatids are joined.
Karyotype
Visual representation of an individual’s chromosomes.
Autosomal Chromosomes
22 pairs in humans, not related to sex determination
Haploid Cells
Contain one set of chromosomes (n)
Cell Cycle
Includes interphase (G1, S, G2) and mitotic (M) phase
Diploid Cells
Contain two sets of chromosomes (2n)
Meiosis
Produces four haploid daughter cells with genetic variability
G0 Phase
Non-dividing state, exits active cell cycle
Cytokinesis
Division of the cytoplasm after nuclear division
Cell Cycle Checkpoints
Regulate cell cycle progression and ensure accuracy
Sexual Reproduction
Involves fusion of gametes for genetic recombination
Synapsis
Pairing of homologous chromosomes in meiosis
Asexual Reproduction
Produces genetically identical offspring
Crossing Over
Exchange of genetic material in prophase I of meiosis
Independent Assortment
Random alignment of homologous chromosomes
Phenotype
Observable traits of an organism
Nondisjunction
Improper separation of chromosomes during division
Gregor Mendel
Father of modern genetics, studied pea plants
Alleles
Alternate forms of a gene at the same locus
Genetic Variability
Occurs through independent assortment, crossing over, and fertilization
Genotype
Genetic makeup of an organism
Codominance
Both alleles contribute to the phenotype in a heterozygote
Monohybrid Crosses
Inheritance of a single trait governed by one gene
Incomplete Dominance
Heterozygous phenotype is intermediate between homozygotes
Loci
Specific positions on chromosomes where alleles are found
Epistasis
One gene’s expression masks or modifies another gene
Sex-Linked Gene
Gene located on a sex chromosome, inherited differently in males and females
Polygenic Inheritance
Trait controlled by multiple genes, leading to continuous variation
SRY Gene
On Y chromosome, initiates male sex determination
Pleiotropy
Single gene influences multiple seemingly unrelated traits
Color blindness
Example of X-linked recessive trait.
X-linked recessive traits
Expressed in males due to single X chromosome.
Cytoplasmic inheritance
Genes located outside nuclear chromosomes.
Hemophilia
Example of X-linked recessive trait.
Genomic imprinting
Gene expression dependent on parent of origin.
Genetic recombination
Exchange of genetic material between chromosomes.
Trisomy
Presence of an additional chromosome.
Monosomy
Absence of one chromosome.
Turner syndrome
Associated with XO chromosome.
Down syndrome
Absence of one chromosome.
Klinefelter syndrome
Associated with XXY chromosome.
Triple X syndrome
Associated with XXX chromosome.
Jacob’s syndrome
XYY syndrome with extra Y chromosome.
Chorionic villus sampling (CVS)
Genetic testing from placental cells.
Genetic counseling
Support for genetic conditions and family planning.
Central dogma of molecular biology
Genetic info flow: DNA to RNA to protein.
Transcription
DNA to RNA synthesis process.
Amniocentesis
Genetic testing from amniotic fluid.
mRNA
Carries genetic info for protein synthesis.
Translation
Decoding mRNA to amino acids for protein synthesis.
Coding strand
Decoding mRNA to amino acids for protein synthesis.
Template strand
Decoding mRNA to amino acids for protein synthesis.
tRNA
Carries amino acids during translation.
rRNA
Structural component of ribosomes.
Prokaryotic transcription
Occurs in the cytoplasm.
Eukaryotic transcription
Occurs in the nucleus.
Amino acids
Building blocks of proteins.
Genetic code
Rules for translating mRNA to proteins.
Codon
Three-nucleotide sequence in mRNA.
Stop codons
Signals termination of translation.
Start codon
AUG codes for methionine and translation start.
Degenerate genetic code
Multiple codons for most amino acids.
Universal genetic code
Shared code across living organisms.
Gene parts
Coding and regulatory regions.
Transcription stages
Initiation, elongation, termination.
RNA polymerases
Enzymes for transcribing DNA into RNA.
Post-transcriptional modifications
Addition of cap and poly-A tail to mRNA.
Exons
Coding regions in mature mRNA.
Introns
Non-coding regions removed during splicing.
mRNA splicing
Removal of introns and joining of exons.
Non-coding DNA
Significant portion in eukaryotic genomes.
Anticodon
Three-nucleotide sequence in tRNA.
tRNA charging
Process of adding amino acid to tRNA.
Aminoacyl-tRNA synthetase
Enzyme transferring activated amino acid to tRNA
Ribosome
Molecular machine with A, P, and E sites
DNA
Deoxyribonucleic acid
Translation stages
Molecular machine with A, P, and E sites
Post-translational modifications
Changes to proteins after synthesis
Chargaff’s rule
Equal A=T, G=C in DNA
Rosalind Franklin
Provided X-ray evidence for DNA structure
Watson and Crick
Proposed DNA double helix structure
Nucleotide
DNA unit with phosphate, sugar, base
Purines and pyrimidines
A,G purines; T,C pyrimidines
Sugar types in DNA
Deoxyribose in DNA, ribose in RNA
Sugar-phosphate backbone
Alternating sugar-phosphate chain in DNA
Base pairing in DNA
A-T, G-C hydrogen bonds in DNA
Anti-parallel strands
Opposite 5’ to 3’ orientation in DNA
Semi-conservative replication
DNA replication yielding two daughter molecules
Helicase
Unwinds DNA double helix
DNA primase
Synthesizes RNA primers for DNA polymerase
DNA polymerase
Adds nucleotides to DNA chain
DNA ligase
Joins DNA fragments during replication
DNA replication components
Template DNA, nucleotides, enzymes
DNA topoisomerase
Relieves torsional strain in DNA replication
Origins of replication
Sites where DNA replication begins
Leading and lagging strands
Continuous and discontinuous DNA synthesis
Replication fork
Region where DNA strands separate
Single-stranded binding proteins
Stabilize unwound DNA during replication
Proofreading by DNA polymerase
Corrects errors in DNA synthesis
Mutation
Change in DNA sequence with various effects
Genomics and Bioinformatics
Study of genomes and computational analysis