The cell cycle Flashcards
What are the phases of the cell cycle?
The cell cycle consists of interphase (G1
What happens during G1 phase?
Cells make RNA
What happens during the S phase of interphase?
DNA synthesis occurs.
What happens during G2 phase?
The cell continues to grow
What is mitosis?
Mitosis is nuclear division
What is cytokinesis?
Cytokinesis is the splitting of the cytoplasm to produce two daughter cells.
What are homologous chromosomes?
Homologous chromosomes are chromosomes with the same genes but different alleles.
What is a chromatid?
A chromatid is one part of a duplicated chromosome.
What is a gene?
A gene is a sequence of bases that determines the sequence of amino acids in a protein.
What happens during prophase?
Chromosomes become visible
What happens during metaphase?
Chromosomes line up across the equator and attach to spindle fibers by their centromeres.
What happens during anaphase?
Chromosomes split at the centromere
What happens during telophase?
The nucleus reforms
How is the mitotic index calculated?
Mitotic index = (Number of dividing cells) ÷ (Total number of cells).
What is apoptosis?
Apoptosis is programmed cell death
What are tumor suppressor genes?
Tumor suppressor genes slow down cell division or trigger apoptosis. If mutated
What are proto-oncogenes and oncogenes?
Proto-oncogenes stimulate normal cell division. Oncogenes are mutated proto-oncogenes that cause uncontrolled cell division.
What is the difference between a mutagen and a carcinogen?
A mutagen induces genetic alterations
How do cancer treatments target the cell cycle?
Some treatments disrupt the cell cycle to slow cancer growth. They target rapidly dividing cells
What are the steps of cell fractionation?
1) Homogenize the sample to release organelles. 2) Filter to remove debris. 3) Centrifuge at low speed to separate heavy organelles. 4) Spin at higher speeds to isolate other organelles.
Why are ice-cold
buffered
What does centrifugation achieve in cell fractionation?
Centrifugation separates organelles based on their size and density.