The Antiglobulin Test, Chapter 5

Question 1

If a tube test is negative for agglutination after AHG is added, what is the next step?

Answer 1

Add check cells.

Question 2

What is the purpose of check cells in the AHG test?

Answer 2

To confirm the validity of a negative result.

Question 3

What does aggluntination of the check cells prove?

Answer 3

It proves that adequate washing was performed to remove unbound antibodies before the AHG reagent was added to the test tube, the AHG reagent was working properly, and that the AHG reagent was added to the test tube.

Question 4

If check cells fail to agglutinate, what is the next step?

Answer 4

The antibody screen must be repeated from the beginning.