Thacker Introduction Flashcards

Lipids, Proteins, Carbohydrates

1
Q

5 pillars of animal health

A

Nutrition, breeding, disease, environment, management

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2
Q

5 functional components of plant/animals tissue

A

Fats, CHOs, Protein, Ash, Water

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3
Q

Absorption

A

Passage of the end-products of digestion from the GI into the body via the blood and lymph

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4
Q

Digestion

A

Taking big particles and making them small by a series of chemical reactions and hydrolysis

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5
Q

Metabolism

A

changes which nutrients undergo between the absorption and excretion as waste products

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6
Q

Anabolism

A

making molecules bigger. uses energy

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7
Q

Catabolism

A

making molecules smaller, gives off energy

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8
Q

purpose of nutrition

A

meet requirements at the lowest cost

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9
Q

(5) What makes a good diet?

A
  1. Contains all the ingredients
  2. All the essential nutrients in the right proportions
  3. Palatable
  4. Economical
  5. No toxic substances
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10
Q

Nutrient

A

a chemical element or compound in the diet of an animal that supports normal life processes (essential nutrients are key)

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11
Q

6 Classes of nutrients

A
  1. Vitamins,
    1. Minerals
    2. Water
    3. CHOs
    4. Protein
    5. Lipids
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12
Q

5 reasons for doing a feed analysis

A
  1. Give a balanced diet
  2. For use in estimating TDN or Net energy
  3. Solve a production problem
  4. Give a market value for feed
  5. Verify a commercial guarantee??
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13
Q

Nutrition

A

Science of how nutrients in the feed are utilized and converted into animal products

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14
Q

Cautions with NRC tables (3)

A
  1. minimum requirements
  2. don’t consider stress, disease or parasite load
  3. sensitive to environmental conditions
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15
Q

6 parts to the Proximate Analysis

A
  1. Moisture content
  2. Ash (minerals)
  3. Crude protein
  4. ether extract (lipid measure)
  5. crude fiber (CHOs)
  6. nitrogen free extract (NFE - for CHOs)
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16
Q

Steps to measure moisture (4)

A

Weigh –> go to 100C in oven —> weigh –> calc (weight after/ beginning weight)

17
Q

Ash - characteristics (5)

A
  • not individual measurement
  • 600C burning
  • P and Se are destroyed
  • measuring is the same procedure as water
18
Q

Wet ashing

A

using HClO (perchloric acid) to burn for P and Se

19
Q

Kjeldahl Process (6)

A

weigh –> Boil in H2SO4 (to get ammonia sulfate) –> Add NaOH (ammonia OH) –> Add water in a known amount of HB (boric) –> Titrate in HCl (measures ammonia) –> multiply by 6.25

20
Q

Flaws in Kjeldahl (2)

A
  1. all nitrogen comes from protein

2. all protein contains 16% N

21
Q

Soxhlet process (4)

A

Weigh —> dehydrate –> extract with petroleum ether (or soxhlet extractor) –> evaporate (whats left is lipid)
High extract = high energy feed

22
Q

Inaccuracies in Soxhlet (2)

A
  1. Accounts for undigestible lipids

2. does not get the individual vitamins (so we expect the in the feeds anyway)

23
Q

Process of measuring CF (4)

A

Weigh –> Boil in H2SO4 (30 min) –> Boil in NaOH (30 min) —> dry and weigh –> residue/beginning weight. This process simply mimicks the stomachs process

24
Q

Van Soest method

A

partitions fiber into soluble and insoluble

25
NDF =
Hemicellulose, Cellulose, and Lignin (hemicellulose is the most indigestible)
26
ADF =
Lignin and Cellulose
27
NFE calculation
NFE = 100 - (H2O, ASH, CF, CP, EE)
28
Advantages of Proximate Analysis (3)
cheap, decently accurate, basis of TDN
29
Amino Acid Analysis Steps (4)
Hydrolysis ---> Separation ---> Detection ---> ID and quantify
30
3 methods of hydrolysis
1. Acid (6 Normals HCl for a day) 2. Basic Hydrolysis (4 Normals NaOH) - doesn't get Trp 3. Performic Acid Hydr. (for Cysteine and Serine) This is just to liberate the AA
31
Acid Hydrolysis doesn't account for
Met, Cys, Trp
32
Basic Hydrolysis doesn't account for
most except Trp
33
Performic acid does account for
Cys, Ser
34
How do you separate after Hydrolysis?
Ion exchange chromotography - in an AA chromatogram
35
Quantifying AA content
take the area under the curve of the Chromatogram
36
What chemicals are used to identify AA?
Ninhydrin, and OPA
37
Steps in Atomic Absoprtion (5)
Ash --> solubilize them (HCl) ---> suck sample into flame (to be in ground state) --> use energy from a hollow cathode lamp (for specific emittance) --> amount of light will reflect concentration
38
Which element is not measured by Atomic Absoprtion and how is it measured?
P, by colorimetric method
39
What does GLC do (gas-liquid chromatography)?
measures individual fatty acids. Shorter ones come off first. Also, double bonds fall off later.