TESTS FOR ENTEROBACTERIACEAE

Question 1

Phenol red

FeSO4

Answer 1

TSI

Yellowing = fermentation
Splitting = gas
Blackening = H2S

Question 2

Bromcresol purple

Answer 2

LIA AND DECARBOXYLASE

Decarboxylation: Purple (- ) Yellow
Deamination: red (-) PURPLE

DECARBOXYLASE: PURPLE

Question 3

Kovac’s reagent

Answer 3

Indole

Red ring

Question 4

Methyl red

Answer 4

MR

red (-) Yellow

Question 5

40% KOH + αnaphthol

Answer 5

VP

red (-) Yellow

Question 6

Bromthymol blue

Answer 6

Citrate AND AMA

BLUE ON BOTH TESTS
(-) GREEN/YELLOW

Question 7

Phenol red

Answer 7

Urease

Pink (-) Yellow

Question 8

ONPG

Answer 8

ONPG

Yellow

Question 9

10% ferric chloride

Answer 9

PAD

green

Question 10

Decarboxylation of lysine,

ornithine, arginine

Answer 10

Decarboxylase

Question 11

Initial ID of Proteus, Providencia

and Morganella

Answer 11

PAD

Question 12

ID late lactose fermenters

Answer 12

ONPG

Question 13

Urease production

Answer 13

Urease

Question 14

Acetate, malonate, acetamide as

source of C

Answer 14

AMA

Question 15

Citrate as source of carbon

Answer 15

Citrate

Question 16

Acetoin production

Answer 16

VP

Question 17

Acid production = pH 4.4

Answer 17

MR

Question 18

detects tryptophanase

Answer 18

Indole

Question 19

Detects ability to degrade lysine

Answer 19

LIA

Question 20

Detects fermentation of lactose,

sucrose, glucose

Answer 20

TSI