Tests For Biological Molecules Flashcards
Test for starch
Add iodine to sample, turn to blue/black.
Test for reducing sugars
Heat with Benedict colour change form blue to green to yellow to orange.
Test for on reducing sugar
You have to hydrolyse bond.
1)take test for reducing.
2)boil with hydrochloric acid
3)cool, and add sodium hydrogencarbonate, to ensure it’s neutralised.
4)test for reducing.
Tests for lipids
Take sample, mix with ethanol and H2O. Filter it , and will become cloudy while emulsion,
Test for biruets
Add iodine turns blue/black if present.
Colorimeter
Shining light through sample.
Place in cuvette, and put in colorimeter.
Plot table. And calibration curve..
Use of biosensors
Take biological substances, and convert to electrical.
Chromotography
1)draw line with pencil and put tiny drop on line.
2)spot solution on pencil dot, wait to dry. Then lower to solvent.
3)cover beaker with watch glass.