Tests Flashcards
What does benedicts test for?
Reducing and non-reducing sugars
Method for testing for reducing? Results if present?
1)place sample in boiling tube
2)add drops of benedicts
3) heay mixture in hot water bath for 5 minutes
RESULTS- red precipitate will form
What would you have to fo before the method for benedicts if the sugar is non reducing?
It would have to be hyrdrolysed by boiling it with dilute hydrochloric acid and them neutralided with sodium hydrogen carbonate
What is the tesy for proteins? Results if positive?
-Biuret solution
- solution will turn from blue to lilac
Test for lipids? Results if positive?
-emulsion test: add ethanol to sample and shake vigourously
- white precipitate forms if lipids present
What is the test for starch? Results if positive?
- iodine solution
- solution turns from orange to black