TEST 5 Flashcards
what is assessed during the oral hygiene assessment
dental biofilm, calculus, and stain
define oral hygiene
the degree to which the oral cavity is kept clean and free of soft and hard deposits
during the clinical exam, examine teeth and tissues for: (4)
- amount of hard and soft tooth deposits
- oral hygiene status
- oral self care effectiveness
- motivation related to oral self care
ACQUIRED ENAMEL PELLICLE
- description
- derivation
- removal method
- translucent, homogeneous, thin, structured film covering and adherent to the surfaces of the teeth, restorations, calculus, and other surfaces
- supragingival: saliva, oral mucosa, microorganisms
- subgingival: gingival crevicular fluid
- toothbrush and appropriate interdental aid such as floss
ACQUIRED DENTIN PELLICLE
- description
- derivation
- removal method
- translucent, two-layer structure, adheres to exposed dentin and restorative materials
- saliva, gingival crevicular fluid, and dentinal fluid
- toothbrush and appropriate interdental aid like floss
MICROBIAL (bacterial) BIOFILM NONMINERALIZED
- description
- derivation
- removal method
- dense, organized bacterial communities embedded in EPS matrix adheres tenaciously to the teeth, calculus, prostheses, and other surfaces in the oral cavity
- colonization of oral microorganisms
- toothbrush and appropriate interdental aid such as floss
MATERIA ALBA NONMINERALIZED
- description
- derivation
- removal method
- loosely adherent, unstructured, white or grayish-white mass of oral debris and bacteria that lies over dental biofilm
- incidental accumulation
- vigorous rinsing and water irrigation can remove materia alba
FOOD DEBRIS NONMINERALIZED
- description
- derivation
- removal method
- unstructured, loosely attached particulate matter
- food retention following eating
- self cleaning activity of tongue and saliva
- rinsing vigorously removes debris
- toothbrushing, flossing, and other aids
CALCULUS MINERALIZED
- description
- derivation
- removal method
- calcified dental biofilm; hard, tenacious mass that forms on the clinical crowns of the natural teeth and on dentures and other oral appliances
- biofilm MINERALIZATION
- see further subtypes
CALCULUS MINERALIZED (SUPRAGINGIVAL)
- description
- derivation
- removal method
- occurs coronal to the margin of the gingiva, is covered with dental biofilm
- source of minerals is saliva
- manual instrumentation
- ultrasonic instrumentation
ACQUIRED PELLICLE
Interface between the _________ and the _________
thin, _________, ___________ film
Forms over__________, existing ________, _________ and _________
Composition:
- primarily __________
- derived from the _____, _________ cells, microorganisms, and _______________
Interface between the TOOTH SURFACES and the ORAL ENVIRONMENT
thin, ACELLULAR NONMINERALIZED film
Forms over EXPOSED TOOTH SURFACES, existing CALCULUS DEPOSITS, RESTORATIONS and PROTHESES
Composition:
- primarily GLYCOPROTEINS
- derived from the SALIVA, ORAL MUCOSA cells, microorganisms, and GINGIVAL CREVICULAR FLUID
acquired enamel pellicle
- clear, transparent and _______ until a _________ has been applied
- pellicle can take on _________ and become gradations of ______, __________, or other colors
- when stained with a _______, pellicle appears _____, with a ________ that contrasts with the thicker, darker staining of _________
- clear, transparent and NOT READILY VISIBLE until a DISCLOSING AGENT has been applied
- pellicle can take on EXTRINSIC STAINS and become gradations of BROWN, GRAY, or other colors
- when stained with a DISCLOSING AGENT, pellicle appears THIN, with a PALE STAINING that contrasts with the thicker, darker staining of DENTAL BIOFILM
acquired dentin pellicle:
occurs on ___________
occurs on DENTIN EXPOSED BY RECESSION
FUNCTIONS OF PELLICLE
- protective
- lubrication
- host defense
- microbial colonization
PROTECTIVE: provides barrier against acids; impacting remineralization and demineralization. May protect against erosion
LUBRICATION: keeps surfaces moist and prevents drying; enhances the efficiency of speech and mastication. May also protect against abrasive damage
HOST DEFENSE: 8% of proteins in the pellicle have antimicrobial functions
MICROBIAL COLONIZATION: pellicle participates in biofilm formation by aiding the adherence of microorganisms
ORAL DEPOSITS: DENTAL BIOFILM
- ___________ of microorganisms that are enclosed in an _____________ (EPS); forms a ________ around microcolonies
- Known as ______, __________, and __________
- Exhibits ________ and ________ properties
- Plays a major role in ______ and ________ of ______ and __________; significant in the formation of __________ (mineralized dental biofilm)
- STRUCTURED COMMUNITY of microorganisms that are enclosed in an EXTRACELLULAR POLYMERIC SUBSTANCE (EPS); forms a SLIME MATRIX/LAYER around microcolonies
- Known as MICROBIAL PLAQUE, DENTAL PLAQUE BIOFILM, and BACTERIAL PLAQUE BIOFILM
- Exhibits SELF-SUSTAINING and PROTECTIVE properties
- Plays a major role in INITIATION and PROGRESSION of DENTAL CARIES and PERIODONTAL DISEASES; significant in the formation of DENTAL CALCULUS (mineralized dental biofilm)
EPS SLIME MATRIX/LAYER (of dental biofilm)
- composed of ________, ________, and other compounds
- _______ biofilm from _________ and ___________
- composed of POLYSACCHARIDES, PROTEINS, and other compounds
- PROTECTS biofilm from HOST’S IMMUNE SYSTEM and ANTIMICROBIAL AGENTS
what are the stages of biofilm formation
- pellicle formation
- initial adhesion/colonization
- maturation
- detachment and disperation
describe the stages
- pellicle formation
- initial adhesion/colonization
- maturation
- detachment and dispersion
PELLICLE FORMATION: formation of a pellicle
INITIAL ADHESION/COLONIZATION:
- attachment of planktonic bacterial cells to the pellicle
- creation of layers of immobile colonies
- EPS slime layer forms:
- stickiness facilitates microbial adhesion
- limits antibiotic therapy/host immune response
- water insoluble
MATURATION:
- later colonizing bacteria attaches
- cell-to-cell communication
- mass/thickness increase
DETACHMENT AND DISPERSION:
- bacteria detaches to relocate and form new biofilm colonies in other areas of the mouth
BIOFILM FORMATION TIME FRAME
days 1-2
- _____ biofilm consists primarily of ________
days 2-4
- ______ dominate while increasing numbers of ________ form and slender rods join the surface of the ________ along with more ___________
- gradually, cocci adhere to _________ in a ________ appearance
- dental biofilm is said to have matured by _____ and is capable of _________
days 5-10
- filaments _______ in number and ________ appears comprised of ____, ________, and _________ with heavy accumulation of _______
- __________ bacteria present
days 10-21
- ________ clinically evident
days 1-2
- EARLY biofilm consists primarily of GRAM-POSITIVE AEROBIC COCCI
days 2-4
- COCCI dominate while increasing numbers of GRAM-POSITIVE FILAMENTOUS form and slender rods join the surface of the COCCI COLONIES along with more LEUKOCYTES
- gradually, cocci adhere to FILAMENTOUS BACTERIA in a “CORN COB” appearance
- dental biofilm is said to have matured by 72 HOURS and is capable of INITIATING THE INFLAMMATORY PROCESS
days 5-10
- filaments INCREASE in number and MIXED FLORA appears comprised of RODS, FILAMENTOUS FORMS, and FUSOBACTERIA with heavy accumulation of LEUKOCYTES
- GRAM-NEGATIVE ANAEROBIC bacteria present
days 10-21
- GINGIVITIES clinically evident
- a freshly cleaned tooth surface immediately develops a thin layer of salivary glycoproteins (the acquired pellicle)
- fibers of proteins, antibodies, salivary enzymes, bacterial debris, and other salivary molecules adhere to the pellicle
- the earliest tooth colonizers are the streptococcus mutans group (s oralis and s gordoni). these bacteria have specific receptors that adhere to the outer pellicle molecules. the streptococci likewise bind to each other, forming the initial base of plaque.
- the next phase involves cell-cell signaling and coaggreation with additional colonists. the most common bacteria to add to the biofilm during this phase are filamentous rods in the genus actinomyces. other species of streptococcus use dietary carbohydrates to secrete glucans that add bulk to the matrix and serve as a source of sugars
- once this initial framework has been laid down, it enters a second phase of aggregation which creates the final dense mat of plaque. Bacteria that colonize at this point are frequently anaerobes such as fusobacterium, porphyromonas, prevotella, vellonella, and treponoma
- see upper left, initial damage to the enamel occurs when streptococci near the enamel surface ferment the sugars in plaque to lactic, acetic, and other acids. when these acids are trapped against the tooth surface and etch through it, a dental caries has developed
…
CHANGES IN MICROBIOFILM
The formation and destructive nature of oral biofilm highlights the need for its _________ by _________
The longer the oral biofilm remains undisturbed, the greater its _____________
Can lead to _____, ______, and eventually _______
The host’s _________ is activated and eventually ________, causing __________ destruction in periodontitis
The formation and destructive nature of oral biofilm highlights the need for its DAILY REMOVAL by MECHANICAL MEANS
The longer the oral biofilm remains undisturbed, the greater its PATHOGENIC OR DISEASE-PRODUCING POTENTIAL FOR THE HOST
Can lead to CARIES, GINGIVITIS, and eventually PERIODONTITIS
The host’s IMMUNE SYSTEM is activated and eventually OVERRESPONDS, causing TISSUE AND BONE destruction in periodontitis
CHANGES IN BIOFILM MICROOGANISMS
- -
Bacteria associated with periodontitis are predominantly ANEROBIC, GRAM-NEGATIVE, and include (but not limited to):
- PORPHYROMONAS GINGIVALIS
- TANNERELLA FORSYTHIA
- PREVOTELLA INTERMEDIA
- AGGREGATIBACTER ACTINOMYCETEMCOMITANS
ASSESSMENT TOOLS REQUIRED
- light
- compressed air
- mouth mirror
- periodontal explorer
- gauze
- disclosing solution
CLINICAL ASSESSMENT OF ORAL BIOFILM
the presence of oral biofilm is most commonly assessed by __________ over the _________
_________ are used to make the oral biofilm _______
the presence of oral biofilm is most commonly assessed by PASSING A DENTAL EXPLORER over the TOOTH SURFACE
DISCLOSING AGENTS are used to make the oral biofilm CLINICALLY VISIBLE