Test #3- Translation Flashcards
What is the function, location and purpose of translation?
- converting genes to protein
- ribosomes
- synthesising proteins
What does mRNA do?
messenger RNA–specifying amino acid sequence of proteins from DNA to ribosomes
What dies tRNA do?
Transfer RNA–translated mRNA codons into amino acids
What does rRNA do?
Ribosomal RNA– catalytic and structural roles in ribosomes
what does the primary transcript do?
precursor to mRNA, rRNA, and tRNA
What does snRNA do?
small nuclear RNA– complexes of proteins and RNA that splice pre-mRNA. Also plays structural and catalytic roles in Spliceosomes
What is the Wobble theory?
that there are 64 codons but only 45 tRNA–possible that the last codon doesn’t need exact protein that it is for
What are the sites of actions in ribosomes?
E-exit
P- peptidyl- tRNA binding site
A- amino acytl-tRNA binding site
what are the 3 steps in Translation?
Start codon (MET)
adds a protein to P each time then it moves over to the left
the release factor binds to the A site
What are the ends of RNA of a polypeptide?
NH2 (first formed)
COOH (last formed)
How is the COOH end made?
the break down of water and the CO-
What are 3 modifications to a functional protein?
1) protein folding
2) post translational modification
3) Signal peptide at N-terminus (NH2) like an address