Test 3 RNAi Flashcards by Joseph Deaton | Brainscape

Q

miRNA gene silencing is done how?

A

Inhibiting translation

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Q

How are siRNAs generated?

A

Dicer makes 2 cuts.

The strands are perfectly complementary.

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Q

How are miRNAs generated.

A

Coded by
Transcribed by RNA pol 2
- Single stranded but forms hairpin with signals for dsRNA-specific nuclease cleavage.
- 5’ Cap and 3’ poly A tails.
Primary miRNA cleaved by DROSHA
Transported out by Exportin5
Then in the cytoplasm it is cleaved by DICER-TRBP makes final cut.
- miRNA 21-23 nucleotides with overhang.

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