TEst 3-9 (Major groups disease causing bacteria) Flashcards

1. List the gram staining and morphological properties of the bacterial genera described in the reading assignment. 2. Use the results from a catalase test to differentiate between the Streptococcus and Staphylococcus genera. 3. Use the hemolysis on blood agar and results from the Optochin and Bacitracin sensitivity tests to differentiate amongst the Streptococcus species. 4. Differentiate amongst the Staphylococcus species based upon results from the coagulase and Novobiocin sensitivity test

1
Q

Gram-positive bacteria

A
  • Aerobic Gram-positive Rods
  • Anaerobic Spore-Forming Gram-positive Rods
  • Gram-positive cocci
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2
Q

Aerobic Gram-positive Rods

A

o Bacillus
o Corynebacterium
o Listeria

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3
Q

Anaerobic Spore-Forming Gram-positive Rods

A

o Clostridium

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4
Q

Gram-positive cocci

A

o Staphylococcus
o Streptococcus
o Enterococcus

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5
Q

Gram-negative bacteria

A
  • Gram-negative Cocci
  • Enteric Gram-negative rods
  • Nonenteric Gram-negative rods
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6
Q

Gram-negative Cocci

A

o Neisseria

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7
Q

Enteric Gram-negative rods

A
o Escherichia
o Campylobacter
o Yersinia enterocolitica
o Salmonella
o Shigella
o Helicobacter
o Vibrio
o Bacteroides
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8
Q

Nonenteric Gram-negative rods

A
o Haemophilus
o Legionella
o Francisella
o Pasteurella
o Brucella
o Rickettsia
o Pseudomonas
o Klebsiella
o Yersinia pestis
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9
Q

Bacteria not usually Gram-stained

A
  • Elementary body/Reticulate body
  • Spirochetes
  • No-cell wall
  • Unique cell wall
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10
Q

Elementary body/Reticulate body

A

o Chlamydia

o Chlamydophila

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11
Q

Spirochetes

A

o Borrelia

o Treponema

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12
Q

No-cell wall

A

o Mycoplasma

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13
Q

Unique cell wall

A

o Mycobacterium

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14
Q

Bacillus anthracis morpology

A
  • Aerobic Gram-positive Rods
  • anthrax- biological warfare/bioterrorism agent
  • morphology: large end to end chains
  • Produces spores
  • No motility
  • Poly-D-glutamic acid capsule; spores
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15
Q

Bacillus cereus morpology

A
  • Aerobic Gram-positive Rods
  • food poisoning
  • morphology: large
  • Produces spores
  • 50% of strains have motility
  • Spores
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16
Q

Corynebacterium diphtheriae morpology

A
  • Aerobic Gram-positive Rods
  • diphtheria
  • morpholoy: Small, narrow
  • No spores
  • No motility
  • Pleomorphic; Chinese characters (snapping fission)
17
Q

Listeria monocytogenes morpology

A
  • Aerobic Gram-positive Rods
  • meningtis - foodborne
  • morpholoy: small
  • No spores
  • tumbling motility
  • Grows at 3-4oC; grows intracellularly
18
Q

Streptococcus - -catalase rxn and morphology

A

-catalase negative
-grow in chains
Gram-Positive Cocci

19
Q

Staphylococcus -catalase rxn and morphology

A

-catalase positive
-grow in large clusters
Gram-Positive Cocci

20
Q

Streptococcus pyogenes-catalase/bacaitracin/optochin rxn and morphology

A
  • Gram-positive cocci
  • hemolysis beta
  • sensitive to bacitracin
21
Q

Streptococcus agalactiae–catalase/bacaitracin/optochin rxn and morphology

A
  • Gram-positive cocci
  • hemolysis beta usually, sometimes gamma
  • resistant to bacitracin
22
Q

Streptococcus pneumoniae-catalase/bacaitracin/optochin rxn and morphology

A
  • Gram-positive cocci
  • hemolysis alpha; Viridans group
  • -resistant to bacitracin
  • sensitive to optochin
  • looks green around colony
  • clinical specimens appear lancet shaped or bullet shaped diplococcus
23
Q

Staphylococcus aureus - coagulase and novobiocin rxn

A
  • coagulase positive

- novobiocin sensitive

24
Q

Staphylococcus epidermidis - coagulase and novobiocin rxn

A
  • coagulase negative

- novobiocin sensitive

25
Q

Staphylococcus saprophyticus - coagulase and novobiocin rxn

A
  • coagulase negative

- novobiocin resistant

26
Q

Clostridium botulinum - morphology, spore formation and motility?

A

Anaerobic Spore-Forming Gram-Positive Rods

MOTILE

27
Q

Clostridium tetani– morphology, spore formation and motility?

A

Anaerobic Spore-Forming Gram-Positive Rods

MOTILE

28
Q

Clostridium perfringens– morphology, spore formation and motility?

A

Anaerobic Spore-Forming Gram-Positive Rods

ONLY CLOSTRIDIUM THAT IS NOT MOTILE

29
Q

Neisseria gonorrhoeae -maltose fermentation test

A
  • Gram-Negative Cocci
  • kidney bean shaped (diploccocus)
  • Maltose NEGATIVE (Can ONLY use glucose)
30
Q

Neisseria meningitidis-maltose fermentation test

A
  • Gram-Negative Cocci
  • kidney bean shaped (diploccocus)
  • Maltose POSITIVE (it can use maltose and glucose)
31
Q

Escherichia - lactose fermentation and H2S production tests

A
  • Gram-Negative Rods
  • YES Lactose Fermention
  • NO H2S gas
32
Q

Salmonella- lactose fermentation and H2S production tests

A
  • Gram-Negative Rods
  • NO Lactose Fermention
  • YES H2S gas
33
Q

Shigella- lactose fermentation and H2S production tests

A
  • Gram-Negative Rods
  • NO Lactose Fermention
  • NO H2S gas
34
Q

Yersinia- lactose fermentation and H2S production tests

A
  • Gram-Negative Rods
  • NO Lactose Fermention
  • NO H2S gas
35
Q

Chlamydia and Chlamydophila (cell wall/acid fast staining/wrights stain/obligate intra-pathogens)

A
  • Bacteria Not Usually Gram Stained

- obligate intra cellular pathogen

36
Q

Borrelia (cell wall/acid fast staining/wrights stain/obligate intra-pathogens)

A
  • Bacteria Not Usually Gram Stained

- Identify with Wright stain

37
Q

Treponema (cell wall/acid fast staining/wrights stain/obligate intra-pathogens)

A

-Bacteria Not Usually Gram Stained

??

38
Q

Mycobacterium (cell wall/acid fast staining/wrights stain/obligate intra-pathogens)

A
  • Bacteria Not Usually Gram Stained

- acid fast staining

39
Q

Mycoplasma (cell wall/acid fast staining/wrights stain/obligate intra-pathogens)

A
  • Bacteria Not Usually Gram Stained

- NO CELL WALL