Test 3 Flashcards
What is sensitivity?
implies detector response with the change in concentration/amount of solute (steepest slope = high sensitivity)
What is universal response?
will respond to any component
What is linear range?
the difference in response for two or more concentrations of a given compound is proportional to the difference in concentration of the two samples
What is good stability?
less baseline noise and less drift
What is short term noise?
when the baseline perturbations have a frequency that is significantly higher than the eluted peak
What is long term noise?
perturbations are similar to that of the eluted peak
What is drift?
perturbations that have frequency smaller than that of the eluted peak
What is background noise?
signal produced by the detector in the absence of a sample or solute
What is reliability?
how rugged the detector is for longer period of use
Which characteristics of detectors are complementary of each other?
High sensitivity —> low background noise, fast response High stability —> low background noise
What is the magnitude of the detector signal measured as?
peak area or peak height
What is detector noise?
the maximum amplitude of the combined short and long term noise measured over a period of ten minutes
What is limit of detection (LOD)?
the concentration in a sample that can be detected but not necessarily quantitated under experimental conditions
How can LOD be improved?
enhancing the analyte signal or reducing noise increasing efficiency increasing the injection volume working at optimum flow rate and temp optimizing MP composition or longer path lengths (HPLC)
What is LOQ?
Limit of quantitation with S/N = 10
