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Chromatography > HPLC > Flashcards

HPLC Flashcards

1
Q

What is the MP reservoir?

A

holds the solvent

solvent is delivered through teflon tubing called the inlet line

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2
Q

What is purpose of the sinker frit or push filter?

A

prevent any dust or particulate matter from entering the pump

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3
Q

Why is He degassing of MP required?

A

presence of oxygen in mobile phase causes bubble formation resulting in air in the flow system and pump pressure will change causing a spike in the chromatogram.

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4
Q

What does the pump consist of?

A

check valves
pulse dampener
pressure transducer
flow controller

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5
Q

What do the check valves do?

A

draws liquid in and out of the solvent chamber

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6
Q

What is the pulse dampener?

A

prevent pulses in the baseline created by pump strokes

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7
Q

What is the pressure transducer?

A

monitor the pressure of the liquid flow

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8
Q

What is the flow controller?

A

controls the liquid flow rate

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9
Q

Titanium pump has ____ degree of inertness and is useful for ____ analysis.

A

greater, biomolecule

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10
Q

What are PEEK and Teflon pumps useful for?

A 
corrosive solutions (HCl) are used as MP
separation of metals
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11
Q

What is mircobore?

A

diameter up to 2mm

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12
Q

What is standard bore?

A

2-12mm diameter
most commonly used
100um/min - 10mL/min FR

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13
Q

What is preparative column?

A

> 10mL/min

requires special pump

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14
Q

What is the double piston pump?

A

two pistons driven in opposite directions
1st draws solvent in
2nd draws solvent out

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15
Q

What is the advantage of the double piston pump?

A

minimizes pulsations

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16
Q

What are the two manually operated injectors?

A

septum mode

valve injection

17
Q

What does the valve injector do?

A

allows reproducible introduction of sample into the pressurized MP without interruption of flow even at high temperature

18
Q

What is the flow direction in the load position?

A

MP bypasses sample loop and flows straight from pump into column

19
Q

What is the flow direction in the inject position?

A

sample is introduced into system

MP passes from pump into sample loop into column

20
Q

What are the benefits of the autoinjector?

A

wide variability in injection volume (0.1uL - 2 mL)
precision is better than or equal to manual injection
high sample throughput and less labor intensive

Chromatography (14 decks)

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