Test 2. Lab 9

Question 1

A_________is anassayto analyze the ability oforgans,cellsortissues to maintain or recoverviability

Answer 1

viability assay

Question 2

*Almar Blue

Answer 2

is a proven cell viability indicator that uses the natural reducing power of living cells to convert resazurin to the fluorescent molecule, resorufin.

The inactive ingredient of alamarBlue® (resazurin) is a nontoxic, cell permeable compound that is blue in color and virtually nonfluorescent.

Upon entering cells, resazurin is reduced to resorufin, which produces very bright red fluorescence

Viable cells continuously convert resazurin to resorufin, thereby generating a quantitative measure of viability—and cytotoxicity.

Question 3

• what is resazurin?

Answer 3

a non-fluorescent indicator dye, is converted to bright red–fluorescent resorufin via the reduction reactions of metabolically active cells. The amount of fluorescence produced is proportional to the number of living cells.

Question 4

• name three advantages of alamarBlue and define 1

Answer 4

Quantitative—provides accurate measurement over time

High sensitivity and linearity—detects as few as 50 cells

Robust performance—highly referenced for cytotoxicity and viability assays

No cell lysis—ideal for use with time course experiments or post-measurement functional assays

Flexibility—can be used with primary cells or cell lines, with adherent cells and cell suspensions

Scalable—mix-and-read, homogeneous assay enhances speed while minimizing effort

Compatiblewith fluorescence- or absorbance-based instrumentation

Economical—requires less reagent to get results comparable to the competition

Safe—Nontoxic, nonradioactive reagent that is safe for the user, the cells, and the environment

Question 5

How does alamar blue work?

Answer 5

The amount of fluorescence or absorbance is proportional to the number of living cells and corresponds to the cells metabolic activity.

Damaged and nonviable cells have lower innate metabolic activity and thus generate a proportionally lower signal than healthy cells.

Question 6

KNOW HOW TO CALCULATE THE PERCENT REDUCTION OF ALAMAR BLUE REAGENT USING ABSORBANCE READINGS

Answer 6

TRY OUT THE QUIZ