test 1 Flashcards
in a closed system, gas content
- cannot change
- no gas fluid interface
an increase in H+ concentration results in
-decrease in pH
a decrease in temperature results in
- increase in solubility
- decrease in PPg
- moves dissociation to the left
- increase in pH
pH of neutrality
-at specific temperatures there is a new pH of neutrality
Henry’s law
Dissolved = Solubility x Partial Pressure
Solubility of CO2 and O2
CO2 @ 37 degrees C = 0.06 mls CO2
/100 mls bld/mmHg
O2 @ 37 degrees C = 0.003 mls O2
/100 mls bld/mmHg
CO2 moved from the lungs to tissue (3)
Dissolved
Bound to hemoglobin
As HCO3- (majority of CO2)
-all three determine CO2 content
how to change the pH of blood in a closed system
1) Change total CO2 content – KEEP TEMP CONSTANT
2) Change temperature – keep total CO2 content constant
3) Change total CO2 content – change temperature
Option one: Increase total CO2 content by ADDING additional CO2
pCO2 will increase
pH will decrease
Option one: Decrease total CO2 content by REMOVING CO2
pCO2 will decrease
pH will increase
Option two: Increase temperature
Drive acid-base disassociation equilibrium to the right
[H+] will increase
pH will decrease
Option two: Decrease temperature
Drive acid-base disassociation equilibrium to the left
[H+] will decrease
pH will increase
Option three: Change total CO2 content and Change temperature
pCO2 will change producing an inverse change in pH
Disassociation equilibrium will change causing a
corresponding increase or decrease in [H+]
Temperature Increases: Gas solubility \_\_\_\_\_\_\_\_\_\_\_\_\_ Gas partial pressure \_\_\_\_\_\_\_\_\_\_\_\_\_ Therefore gas pressure is \_\_\_\_\_\_\_\_\_\_\_\_\_ than the starting gas pressure Equilibrium of the water dissociation shifts to the \_\_\_\_\_\_\_\_\_\_\_\_\_ Therefore, pH will \_\_\_\_\_\_\_\_\_\_\_\_\_
Gas solubility decrease
Gas partial pressure increase
Therefore gas pressure is higher than the
starting gas pressure
Equilibrium of the water dissociation shifts to the
right
Therefore, pH will decrease
Draw sample
Need: waste syringe, sample syringe, cup of ice (??)
Draw from patient
Draw from ECC
Remove all air from sample – Cap tightly – Place in cup of ice
Blood Gas Determination Via Lab
Blood from sample injected into analyzer
Analyzer WARMS sample to 37o C
Analyzer determines the pO2, pCO2, and pH at 37o C
uncorrected gases
Report gas values determined at 37oC
corrected gases
Report gas values measured at 37oC but corrected back to actual body temperature
Patient temp is 25o so analyzer will WARM the sample. pH, CO2, and O2 will be
pH will be lower than expected (0.0147 per degree)
CO2 will be higher than expected
O2 will be higher than expected
On bypass, you must look at the temperature corrected
blood gases to correctly treat the patient’s what
pO2
where does most of the acid-base buffering in the body happen
-in the blood
primary blood buffering system for maintaining a constant ratio of OH- to H+ (16:1) due to the changes in temp is
- Imidazole moiety of the amino acid histidine
- found on most blood born proteins
what is controled by alpha stat and pH stat management of blood gases
- pH and pCO2
- arterial pO2 MUST be temperature corrected
how do you increase pCO2
increase CO2 content
In a normal human, what stays constant
CO2 content
intracellular pH (7.0)
- much lower than the blood pH
- more water inside the cells
as temperature increases, what happens to pH
pH decreases
pH varies from organ to organ. Why?
-temperature of each organ is different therefore changing the pH form organ to organ
Advantages of keeping CO2 content constant
Maintains constant pH difference across cell membranes
Maintains constant ratio of negative to positive charges on proteins
Allows enzyme systems to work at optimal level regardless of temperature
what stays constant and what changes for pH stat
- constant- pH
- changes- temperature CO2 content
what stays constant and what changes for alpha stat
- constant- CO2 content
- changes- temperature and pH