test 1

Question 1

what does chemotrypin cleave

Answer 1

aromatic aa

Question 2

what does trypsin cleave

Answer 2

R and K AA basic AA

Question 3

what does it mean when pH < pka

Answer 3

acidic conditions means its protonated because acids want to donate a hydrogen

Question 4

what does it mean when pH > pka

Answer 4

basic conditions, deprotonated because bases want to take a hydrogen

Question 5

gel electrophoresis is helps separate what

Answer 5

AA’s based on size, large molecules get trapped while small ones are able to pass through

Question 6

ion columns help separate what

Answer 6

AA’s that have a certain charge

Question 7

If the pI is greater than the pH, the molecule will

Answer 7

have a positive charge.

Question 8

cation column traps what

Answer 8

positive AA since column is negative

Question 9

anion column traps what

Answer 9

ngative AA since column is positive

Question 10

if pi is less than pH, the molecule will

Answer 10

have a negative charge

Question 11

when pi=ph

Answer 11

molecules will precipitate out

Question 12

ph=pka when

Answer 12

when at half the equivalent of base

Question 13

what property accounts for hydrophobic effect and how do it do this?

Answer 13

hydrophobic effect and it oil decreases entropy while water increases entropy

Question 14

for size exclusion chromatography, what occurs

Answer 14

large leave first