terms

Question 1

define „polycistronic vector“

Answer 1

vector the coexpresses multiple genes from a single transcript

Question 2

define „retroviral vector“

Answer 2

vector that integrates the genetic material into host DNA (stable transduction)

Question 3

transfection vs transduction

Answer 3

transfection: uses chemical or non-chemical methods to deliver genetic material without using a virus

transduction: involves the use of a viral vector to transfer DNA into cells

Question 4

define „floxing“

Answer 4

Floxing refers to the insertion of a DNA sequence (which is said to be floxed) between two LoxP sequences, creating an artificial gene cassette that can be conditionally deleted (KO), translocated, or inverted in a process called Cre-Lox recombination

Question 5

pKA

Answer 5

• negative base 10 log of acid dissociation constant Ka
• indicates strength of an acid
• lower pKa value indicates a stronger acid

Question 6

Chi2 test

Answer 6

used to compare two categorical variables (think frequency or named things without numerical values)

Question 7

ANOVA test

Answer 7

analysis of variance, use for 3+ groups to check for variance between them, takes into account variance within a group and variance between groups,

ANOVA is a statistical test used to analyze the difference between the means of more than two groups.

Question 8

ANOVA test

Answer 8

analysis of variance, use for 3+ groups to check for variance between them, takes into account variance within a group and variance between groups

Question 9

two-way ANOVA

Answer 9

A two-way analysis of variance (ANOVA) is a statistical test used to determine the effect of two nominal predictor variables (two independent variables) on a single, continuous outcome variable (dependent variable).

A two-way ANOVA analyzes the main effect of each of the independent variables on the expected outcome, in addition to the variables’ relationship to each other—if there is any interaction between the independent variables.

https://www.investopedia.com/terms/t/two-way-anova.asp

Question 10

What online tools help you decide on which antibody to use?

Answer 10

• CiteAb (find the most highly cited Abs)
• YCharOS (AB Characterization through Open Science, pronounced “Icarus”: aim is to characterize commerically available research antibodies for every human protein)
• RRID: Research Resource Identifiers: one single identifier for specific antibodies/reagents

Question 11

What is CyTOF? How does it work?

Answer 11

Cytometry by time of flight. Mass spec based cell property characterization. IDEA: Put heavy metals (chelate lanthanide) to antibody of interest, nebulize cells (and dry with heated argon gas), ignite with argon plasma torch, ion cloud is then sent through quadrupole mass spec, heavy ions go through and can be quantified by time of flight.