term need to remember for application genetic

Question 1

selection

Answer 1

a process that only allow for the survival of mutant with the desire characteristic

Question 2

Screening

Answer 2

-investigation of a large number of colony for a desired mutatant

Question 3

Auxotroph

Answer 3

-mutant that can grow in rich medium but no longer on minimal medium
-identified by screening

Question 4

Utilisation Mutants

Answer 4

-mutant that can be grown on medial medium if certain chemical are not present
-identify by screening

Question 5

Resistant mutant

Answer 5

Mutants can be isolated which are resistant to the toxic effect of a particular compound.

Question 6

minimal medium)

Answer 6

consist of a simple source of carbon and energy such as glucose, nitrogen such as ammonia salt w/ salt and trace element

Question 7

Rich medium

Answer 7

containt all the amino acids, lipids, nucleosides and vitamins needed for growth

Question 8

what is the name of two approaches to isolate genes from a genome

Answer 8

1/ genomic DNA (gDNA) : Cleave DNA into manageable fragments and identify the DNA segment containing the segment of interest
2/ Screen complementation : Generate cDNA from mRNA and identify the cDNA segment of interest

Question 9

gDNA libary

Answer 9

-Use recombinant DNA techniques to generate a collection of cloned DNA fragments (library)
-each library clone has a different fragment of DNA in the vector
-screen the entire clone collection to find the gene of interest

Question 10

step in constructing a genomic library indepth

Answer 10

1/ Extract genomic DNA from any tissue
2/ cleave genomic DNA with restriction enzyme into small fragment
3/ ligate DNA into suitable vector which would introduce it into a host
-libary would include all genes that are present in the genome even non-transcribe region

Question 11

Constructing a C library

Answer 11

1/ extract all RNA from a tissuse
2/ Using reverse transcriptase, convert the mRNA into complementary DNA (cDNA)
3/ligate into a vector and insert
-contain only transcribed gene. does not contain non transcribe factor

Question 12

how to ensure Fuctional complemetation in libary clone in diploid stage

Answer 12

identify genes that encode unknown products some key requirement:
1/ Library vector must be capable of being maintained in the target organism
2/ Clones must have intact regulartory sequences to ensure expression in host cell
3/ a recessive mutation will be distinguishable