Technology Flashcards
many histological stains are adapted from
textile dyes
dyes consist of
salts that dissociate in aqueous solutions to form two ions; one coloured and one acid or base
basophilic ions
attract basic ions
things basophilic ions attracted to
nucleic acids; ribosomes
acidophilic ions
attract acidic ions
acidophilic ions attracted to
cytoplasmic proteins; cytoplasm
most common dyes in histology
hematoxylin; eosin; H and E; Nissl; trichome;
hematoxylin oxidation produces
basic blue dye
Eosin is
Acidic dye; pink to red;
Eosin dyes
cytoplasm/extracell proteins
hematoxylin stains
Nuclei, RER
best general purpose histo stain
H&E
Nissl stain
basic dye
Nissl stains what parts
Nuclei, ribosomes,
Nissl best for
Neurons; Ribosomes