Technology Flashcards

1
Q

many histological stains are adapted from

A

textile dyes

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2
Q

dyes consist of

A

salts that dissociate in aqueous solutions to form two ions; one coloured and one acid or base

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3
Q

basophilic ions

A

attract basic ions

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4
Q

things basophilic ions attracted to

A

nucleic acids; ribosomes

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5
Q

acidophilic ions

A

attract acidic ions

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6
Q

acidophilic ions attracted to

A

cytoplasmic proteins; cytoplasm

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7
Q

most common dyes in histology

A

hematoxylin; eosin; H and E; Nissl; trichome;

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8
Q

hematoxylin oxidation produces

A

basic blue dye

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9
Q

Eosin is

A

Acidic dye; pink to red;

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10
Q

Eosin dyes

A

cytoplasm/extracell proteins

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11
Q

hematoxylin stains

A

Nuclei, RER

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12
Q

best general purpose histo stain

A

H&E

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13
Q

Nissl stain

A

basic dye

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14
Q

Nissl stains what parts

A

Nuclei, ribosomes,

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15
Q

Nissl best for

A

Neurons; Ribosomes

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16
Q

Trichome stains

A

Multiple dyes mixed together

17
Q

Most common Trichome stain

A

mallory

18
Q

Using Trichome stains, what stains blck/red/blue?

A

Nuclei = black
Cytoplasm/keratin/muscle = Red
Connective Tissues = blue

19
Q

types of stains using chemical reactions

A
  1. periodic acid schiff reaction
  2. elastin stains
  3. reticular fibre stains
20
Q

periodic acid schiff reaction stain

A

stains carbs red/purple; shows glycogen, cell membrane,

21
Q

in the basement membrane, what component does PASR stain?

A

proteoglycan in BM and ground substance

22
Q

If a cell is stained dark, it means

A

it is secreting protein

23
Q

5 types of structures at the histological level

A

round; tube; sheet; partitioned; cord-like;

24
Q

acini is

A

secretory hole thing

25
Q

what is a partitioned structure

A

have internal divisions marked by connective tissue septae

26
Q

typed of sectioning

A

cross; oblique; longitudinal