Technology Flashcards
many histological stains are adapted from
textile dyes
dyes consist of
salts that dissociate in aqueous solutions to form two ions; one coloured and one acid or base
basophilic ions
attract basic ions
things basophilic ions attracted to
nucleic acids; ribosomes
acidophilic ions
attract acidic ions
acidophilic ions attracted to
cytoplasmic proteins; cytoplasm
most common dyes in histology
hematoxylin; eosin; H and E; Nissl; trichome;
hematoxylin oxidation produces
basic blue dye
Eosin is
Acidic dye; pink to red;
Eosin dyes
cytoplasm/extracell proteins
hematoxylin stains
Nuclei, RER
best general purpose histo stain
H&E
Nissl stain
basic dye
Nissl stains what parts
Nuclei, ribosomes,
Nissl best for
Neurons; Ribosomes
Trichome stains
Multiple dyes mixed together
Most common Trichome stain
mallory
Using Trichome stains, what stains blck/red/blue?
Nuclei = black
Cytoplasm/keratin/muscle = Red
Connective Tissues = blue
types of stains using chemical reactions
- periodic acid schiff reaction
- elastin stains
- reticular fibre stains
periodic acid schiff reaction stain
stains carbs red/purple; shows glycogen, cell membrane,
in the basement membrane, what component does PASR stain?
proteoglycan in BM and ground substance
If a cell is stained dark, it means
it is secreting protein
5 types of structures at the histological level
round; tube; sheet; partitioned; cord-like;
acini is
secretory hole thing
what is a partitioned structure
have internal divisions marked by connective tissue septae
typed of sectioning
cross; oblique; longitudinal
