Techniques for Protein Isolation

Question 1

is one of the most common methods by which proteins are isolated from a mixture of soluble substances

Answer 1

Protein Precipitation

Question 2

Two commonly used protein precipitation

Answer 2

a. Salting-out process
b. Isoelectric precipitation

Question 3

Usually precipitated by saturating the protein with ammonium sulfate or magnesium sulfate.

Answer 3

Salting-out process

Question 4

Salting-out process by saturating protein with what?

Answer 4

Ammonium sulfate or magnesium sulfate

Question 5

pH is the basis of the isolation technique called?

Answer 5

Isoelectric precipitation

Question 6

Solubility of the protein decreases as the ionic strength _____ and the protein eventually ____ ____.

Answer 6

• increases
• precipitates completely

Question 7

What are the two items that we tested to isolate their protein?

Answer 7

Egg and Milk

Question 8

Casein is from

Answer 8

Milk

Question 9

egg albumen is from

Answer 9

egg

Question 10

Formula for calculating yield of egg white albumin

Answer 10

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