Proteins and denaturing agents

Question 1

is the sequence of amino acids

Answer 1

Primary structure

Question 2

Is usually the state in which the protein is most active and functional

Answer 2

Native conformation

Question 3

Proteins are held in their native conformation by what?

Answer 3

combination of forces: such as:
1. hydrogen bonds
2. ionic interactions
3. disulfide bridges
4. hydrophobic interactions.

Question 4

Changing the conformation of a protein either temporarily or permanently by disrupting
these forces

Answer 4

Denaturation

Question 5

What does denaturation do in the protein activity?

Answer 5

results in a loss of protein activity

Question 6

What does Disruption of secondary and tertiary structure causes?

Answer 6

Changes in solubility
frequently results in the formation of solid in the solution

Question 7

Reagents or conditions that can cause denaturation are called?

Answer 7

Denaturing agents

Question 8

Give the examples of Denaturing agents?

Answer 8

a. Heat
b. pH changes
c. Alcohol
d. heavy metal salts

Question 9

can supply kinetic energy to protein molecules, causing their atoms to vibrate more rapidly.

Answer 9

Heat

Question 10

What does heat disrupt?

Answer 10

Weak forces such as hydrogen bons and ionic interactions

Question 11

Example of heat denaturation

Answer 11

cooking an egg