Techniques

Question 1

Fluorescent In Situ Hybridization

Answer 1

This is a method to detect a specific segment of DNA. This is brought about when DNA in the chromosomes are exposed to a fluorescently labeled piece of DNA that binds to the matching sequence.

Question 2

Confocal Microscopy

Answer 2

This is an optical imaging technique to improve resolution and contrast of a micrograph by using a spatial pinhole to block out-of-focus light in image formation.

Question 3

Photobleach

Answer 3

To quench a fluorophore used in Confocal Microscopy

Question 4

Fluorescin Derivatives

Answer 4

These dyes have high absorpitivity, high fluorescin quantum yield and high water solubility.