TCR recognition

Question 1

MHC Subunit recognized by Vb

Answer 1

a2 (general docking)

Question 2

MHC subunit recognized by Va

Answer 2

a1 (general docking)

Question 3

NKT TCR docking

Answer 3

At the end of and paralell too the CD1-Ligand complexess

Question 4

NKT TCR CDR1a

Answer 4

Reco

Question 5

CDR3b, NKT TCR

Answer 5

Highly variable

Question 6

Va14 D0b

Answer 6

D011.10 TCR has a beta chain that is Vb8.2. This pairs with the VaChain to recognize CD1d lipid antigen from James’ 2007 immunity paper

Question 7

Va14iD0b Tetramer recogniztion

Answer 7

NKT TCR from James’ 2007 immunity paper

Binds OCH9, aGC and PBS57

Question 8

Va14 D0b Lipid antigen responses

Answer 8

from James’ 2007 immunity paper - expressed in a hybridoma

Respond to aGC, GSL-1 and IGb3

Question 9

GSL1

Answer 9

Natural antigen for CD1d:

bacterial a-galacturonic acid–containing glycosphingolipid GSL-1’

Question 10

CDR1 swap substitutions

Answer 10

Va14-Ja18 is a type 1 TCR, Type II TCRs commonly express Va3.2 and can also express Va1 or Va4
Switched out the CDR1 of Va14 with CDr1 from type II TCRs
Can’t bind tetramer. This suggests that CDr1 of Va14 is uniquely required for CD1d recognitionn