targeting of lysosomal enzymes to the lysosomes Flashcards
Where is the lysosomal enzyme synthesized?
Rough ER
When the lysosomal enzyme is synthesized, what is added?
Carbohydrate (mannose)
The protein is packaged into a vesicle and transported to the ___-golgi network
cis
Manose is ____ on the ___ carbon of the manose tree by sequential activity of 2 enzymes resulting in ____
phosphorylated, 6th, mannose 6 phosphate (M6P)
What does M6P bind to?
M6P receptor
The tagged enzymes are…
packaged in transport vesicles
Vesicle fuses with..
endosome
What does a low pH of endosome cause?
dissociation of the enzyme from the receptor (decreased binding affinity at low pH)
What happens with the empty M6P receptors?
They are recycled back to the trans Golgi network
Endosome matures into a …
lysosome
Low pH of lysosome is maintained
by activity of
a H+ ATPase
