T1- Microscopy Flashcards
1
Q
What is the object?
A
The real object or sample that you’re looking at.
2
Q
What is the image?
A
The image that we see when we look down a microscope.
3
Q
What is magnification?
A
Magnification is how many times larger the image is than the object.
4
Q
What is the equation for magnification?
A
magnification = Image size ÷ object size
5
Q
What are the positives and negatives of a light microscope?
A
- Small and easy to use
- Relatively cheap
- Resolution is limited to 0.2 μm
- Any details less than 0.2 μm will appear blurry
- Can’t study subcellular structures
6
Q
What are the positives and negatives of an electron microscope?
A
- Hard to use
- Expensive
- Uses electrons instead of light
- Maximum resolution is 0.1 nm
- 2000 x better resolution than light microscopes.
- Can be used to study subcellular structures.
7
Q
How do you get from micrometres (μm) to millimetres (mm)?
A
Divide by 1000
8
Q
How do you get from micrometres (μm)
to nanometres (nm)?
A
Multiply by 1000
