Systems for Detection of Pathogens I Flashcards
What is Taxonomy?
the science of naming and classifying organisms
Why is taxonomy important? Why is naming a pathogen insufficient?
Taxonomy allows a better understanding of relationships among species
-a name only implies capacity for pathogenesis and doesn’t include virulence
Clinical importance of taxonomy
Taxonomy is important so we can identify pathogens which are closely related to each other so that tests can be decided on and be very specific rather than have an overall generalisation.
What is a pathogen?
A microbe CAPABLE of causing disease
-don’t have to cause disease
Different types of pathogens
Commensal Non-Pathogen (in host)
Zoonotic Non-Pathogen (in carrier)
Commensal Opportunist (in host)
Commensal Non-Pathogen (in host)
· Present but not capable of causing disease in the host
· E.g. E.coli
· E.g. Bacteroides thetaiotaomicron ‘good bacteria’
Zoonotic Non-Pathogen (in carrier)
· Present but only capable of causing disease in another host
· E.g. E.coli O157:H7 is subclinical cattle (no disease in cattle, but if you eat meat with this toxin you will be ill)
Commensal Opportunist (in host)
· Present and capable of causing disease in the host but only in certain circumstances (e.g. when immunocompromised)
· E.g. Bacteroides fragilis
· E.g. Coagulase Negative Staphylococcus (CNS)
Requirements when sampling a pathogen
Sterile sites must be free from contamination
>E.g. skin flora in blood cultures
Non-sterile sites require decontamination of normal flora
>E.g. faeces, mouth, skin
Samples with high volume or relatively low infected pathogen load require concentration (centrifugation, filtering)
>E.g. CSF, Ascites, 24 hr urine
Do we have to culture organisms to show/detect it is present?
- It is useful to culture the organism to prove its essence, but it is not essential
- It can show that the sample is there and growing by enrichment, purification and amplification of the organism
- Do not need to culture if you already know that the organism is there
Methods used to detect pathogens include…
- Microscopy
- Classical Culture and Identification
- Virology
How is microscopy used to detect pathogens?
Direct Light Microscopy
-used to detect big organisms (bacteria)
Direct Electron Microscopy
- used to detect small organisms (viruses)
- useful in identifying shapes of viruses
Organisms seen under light microscope
Trichomonas vaginalis (STD)
· Swims
· Easy to see- take a vaginal sample which can be seen immediately under the microscope
Schistosoma Mansonii
- Found in kidneys, damaging kidney tubules
Entamoeba Histolytica
Strongyloides (thread worm)
· Easy to detect
· Used to pull the sellotape on the anus and the worm would lay eggs on it. Sellotape then put under microscope
Method of identifying bacterium under light microscope
Gram Staining:
· identify the type of bacteria (gram negative or gram positive)
· look at the shape (spirals, rods, coccus, bacillus) and features on the bacteria
· see whether a bacterium has a capsule or not. A capsule is an important feature because capsulated organisms are more likely to be pathogenic
· see whether bacterium contains any spores
Organisms seen under electron microscope
- Rotavirus (Reovirus) from Faeces
- Rabies (Lyssavirus) from Brain tissue
- Hepatitis B (Hepadnavirus) from Liver
- Tonsillitis (Adenovirus) from Nasal Secretion