Synaptic Transmission Flashcards
What is the difference between electrical and chemical synapses?
electrical synapse:
- extremely fast
- bi-directional
- one-to-one
- exchange of small molecules
chemical synapse:
- a bit of a delay
- one-directional
- tuneable transmission
- variety of neurotransmitters
What is the contact point called between two neurons?
The synapse
What is the function of an electrical synapse and a chemical synapse?
electrical synapse: synchronisation of cells in a network, fast responses
chemical synapse: regulated activity, plasticity
name 2 different neurotransmitter receptors and whether they are ionotropic or metabotropic
- Ligand-gated ion channels (ionotropic)
- G-protein-coupled (metabotropic)
What properties should a neurotransmitter have?
- should be present/ stored in the presynaptic neuron
- should be released upon depolarisation and calcium influx
- should be only temporary present outside the cell, the signal must be stopped
- should be specifically detected by receptors on the postsynaptic neuron
How is synaptic transmission through chemical synapses tuneable?
- amplification of signals
- suppression of signals
- alteration of signals
(plasticity)
Where do the neurotransmitter vesicles originate from?
the RER and the Golgi
What is the main difference between small clear core vesicles vs dense core vesicles?
SV are recycled, whereas DCV are not as quick
Why are SV recycled?
Neurotransmitter vesicles can travel a distance of 40 cm/day which means it would take 3 days before the neurotransmitter vesicle from the neuron cell body has reached the synapse
What is a synapse?
A contact point between two neurons
Explain the experiment of Heuser and Reese in which they showed that neurotransmitter vesicles are recycled.
They labeled the outside of the neurons with a dark colour. Then they watched whether the colour would be seen inside of the synapse
Name the different stages of fusion of synaptic vesicles and what happens
- Docking; attachment of the vesicle to the release site
- priming; vesicles are prepared for fast release.
- calcium sensing: calcium influx triggers the fusion of the synaptic vesicle membrane with the plasma membrane, releasing the neurotransmitters into the synaptic cleft.
name the key proteins of the fusion of synaptic vesicles and whether they are part of the SV or the plasma membrane
SNARE
- Synaptobrevin on the SV
- Syntaxin and SNAP-25 on the plasma membrane
Calcium sensor
- synaptotagmin on the SV
Name the different steps in the fusion of synaptic vesicles
- synaptobrevin on the vesicle make a connection with Syntaxin and SNAP-25 on the plasma membrane
- these will form a complex and the vesicle will dock
- Synaptotagmin on the SV will bind to the SNARE complex
- the negative charge of the phospholipids of the 2 membranes is a repulsive barrier for fusion
- Entering CA2+ from the plasma membrane binds to synaptotagmin, leading to a more positive charge
- membranes come together and fusion starts
Synaptic vesicle fusion is coupled to ….. influx
Synaptic vesicle fusion is coupled to CALCIUM influx
How does an ionotropic receptor work?
Upon binding of the selective neurotransmitter, this receptor increases the
permeability of the plasma membrane of specific ions. This can result in an ionic
current
What is Ohm’s law
V (Voltage)
————–
I (current) * R (resistance)
What is the formula to calculate the current of an ion?
C ion = g x (V m - E rev)
C ion : current of an ion
g: permeability (0 or 1)
V m: membrane potential
E rev: reversal potention of an ion
What is the equilibrium potential?
A balance between the diffusion forse and electrical forse. No net movement of K+ ions, electrochemical equilibrium
How do you calculate the driving force of an ion?
Vm – E rev ion
V m: membrane potential
E rev: reversal potention of an ion
What does positive\ negative EPC mean?
End-plate current
-Positive EPC is a loss of positive ions or a
gain of negative ions to the cytoplasm, e.g.
the influx of Cl - at 0V .
-Negative EPC signifies a gain of positive
charge or a loss of negative charge to the
cytoplasm of the cell,
e.g. the influx of Na+ at 0V
What happens to the reversal potential if you elevate the extracellular concentration of K+?
Higher external shifts reversal potential to the right. A decreased concentration gradient outward for K+ thus the reversal potential becomes more positive
Name excitatory neurotransmitters and channels
glutamate, Na+, Ca2+ channels
Name inhibitory transmitter and channels
Gaba and CL- channels
