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Microbiology Lab Final > SWI > Flashcards

SWI Flashcards

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Biology 101 flashcards
1
Q

ESKAPE pathogen

A

Enterococcus faecium

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2
Q

ESKAPE pathogen

A

Staphylococcus aureus

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3
Q

ESKAPE pathogen

A

Klebsiella sp.

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4
Q

ESKAPE pathogen

A

Acinetobacter baumannii

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5
Q

ESKAPE pathogen

A

Pseudomonas aerugirosa

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6
Q

ESKAPE pathogen

A

Enterobacter sp.

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7
Q

Autoclave conditions

A

15psi, 121oc

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8
Q

what is the cycle of pcr

A

denaturation; annealing (priming); extension

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9
Q

what fluorescent agent is used in electrophoresis

A

ethidium bromide

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10
Q

chromatogram

A

graphical representation of peaks in fluorescence in a sequencing gel

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11
Q

biology workbench

A

web based tool for analysis of protein and nucleic acid sequences

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12
Q

BLAST

A

tool for comparing primary biological sequence information

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13
Q

genbank

A

website, collection of all publically available nucleotide sequences and their protein translations (includes blast)

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14
Q

clustal

A

multiple sequence alignment program for nucleotide/protein sequences

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15
Q

what are the pcr reagents

A

CoralLoad PCR Buffer; Q Solution; MgCl2; dNTP mix; 27f Primer; 1492r Primer; Taq DNA Polymerase; sterile water

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Microbiology Lab Final (10 decks)

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