Stains

Question 1

Name primary stain, mordant, decolorizing agent, counterstain and additional information

Simple Stain

Answer 1

any basic or acid dye; no mordant, no decolorizing, no counterstain

Question 2

Name primary stain, mordant, decolorizing agent, counterstain and additional information

Gram Stain

Answer 2

Crystal Violet; iodine; acetone/alcohol; safranin; G+ are purple G- are pink

Question 3

Name primary stain, mordant, decolorizing agent, counterstain and additional information

Endospore Stain

Answer 3

Malachite Green; no mordant; room temp water; safranin; stem is needed to apply primary stain

Question 4

Name primary stain, mordant, decolorizing agent, counterstain and additional information

Acid-fast Stain

Answer 4

Carbol Fuschin; no mordant; acid-alcohol; methylene blue; one technique requires steam

Question 5

Name primary stain, mordant, decolorizing agent, counterstain and additional information

Nigrosin Negative Stain

Answer 5

Nigrosin; no mordant; no decolorizing; no counterstain; dye is spread with a slide, air dries

Question 6

Name primary stain, mordant, decolorizing agent, counterstain and additional information

Anthony’s Capsule Stain

Answer 6

Anthony’s Crystal Violet; no mordant; 20% copper sulfate; no counterstain; cv is less conc, grown in litmus milk, capsule not stained