Supplementary Questions

Question 1

If 1 mL of 1/10 diluted saliva was added to 4.5 mL of starch, and the achromic point was reached in 6 minutes, the number of U/mL for undiluted saliva would be calculated how? (give formula)

Answer 1

[enzyme] = 1 U/mL(4 min/achromic point actual}(inverse dilution factor)

Question 2

Why is it not meaningful to compare heat treated DNA to DNase treated DNA?

Answer 2

DNase cleaves dsDNA into smaller fragments, heat denatures into long ssDNA. Both treatments produce different structures that cannot be compared

Question 3

A titration curve that is indicative of a triprotic molecule is indicative of what sort of amino acid?

Answer 3

One that is basic or acidic (Eg. three dissociable groups)

Question 4

What pKa2 from a titration curve is indicative of an acidic amino acid?

Answer 4

pKa 4

Question 5

How many nkat equals 1 U?

Answer 5

16.67

Question 6

Which direction would LDH catalyze pyruvate/lactate conversion in vigorously contracting skeletal muscle?

Answer 6

Pyruvate to lactate

Lots of NADH

Question 7

Which direction would LDH catalyze pyruvate/lactate conversion in well oxygenated cells of cardiac muscle?

Answer 7

Lactate to pyruvate

Lots of NAD+