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Intro to MLT 110 study guide 3 > study guide 3 > Flashcards

study guide 3 Flashcards

1
Q

what removes dried oil from the lens the best?

A

Xylene

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2
Q

solid cone of light delivered to the specimen by the condenser and gathered by the objective

A

mumerical aperture on the microscope

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3
Q

ability of the scope to reveal or seperate fine detail

A

resolution

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4
Q

the distance from the center of the objective to the top of the specimen also called the focal length

A

working distance

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5
Q

imperfect refraction or focalization of a lens; inability to bring light rays to a single focus

A

abberation

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6
Q

reflects the beam of light upward

A

mirror

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7
Q

directs and focuses the light from the light source up to the specimen

A

condenser

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8
Q

total magnification of a specimen on a microscope

A

ocular times objective

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9
Q

what are the three most common objectives used in the clinical lab and what are they otherwise known as

A

10x-low 40-45x- high 100x-oil

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10
Q

what magnification are most occulars

A

10x

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11
Q

uses daylight or light bulb with a compound lens system

A

bright field microscope

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12
Q

special condenser makes background appear dark while the specimen is light. The condenser allows light waves to cross on the specimen instead of through it.

A

dark field microscope

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13
Q

equipped with a UV light source and special filter to allow shorter wave lenth.

A

flourescent microscopy

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14
Q

special diaphragm into or below the condenser to view unstained structures

A

phase contrast microscope

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15
Q

uses a special filter that takes ordinary light waves and allows only light waves of one orientation(north-south) to pass through the filter and reach the specimen. To illuminate objects that can rotate light.

A

polorized microscopy

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16
Q

substitution of an electron bean for light rays to achieve greater resolution

A

electron microscope

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17
Q

the light circle you see through the microscope

A

field of view

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18
Q

what is a MSDS and what kind of information can a lab employee get from it?

A

material safety data sheet

describes hazards, safe handling, storage and disposal of hazardous chemicals

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19
Q

what do the colors of the diamond hazard symbol give you information about?

A

red-fire hazard yellow-reactivity blue-health hazard white-specific hazard rate them 0-4

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20
Q

treat all specimens as potentially infectious

A

standard precautions

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21
Q

class A

A

anthrax, botulism, plague, small pox, tularemia, ebole

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22
Q

class B

A

bacterial, C perfringens, ecoli 105H7, salmonella, shigella,

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23
Q

Class C

A

emerging pathogens that could be engineered

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24
Q

what is the single most effective way to reduce the possibility of contaminating yourself?

A

washing your hands

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25
Q

How long is HBV stable in blood products at 25 degrees C

A

7 days

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26
Q

where should contaminated materials be disposed?

A

bio hazard storage

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27
Q

caustic chemicals on skin

A

skin washed off with water for 5 mins immediately after exposure.

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28
Q

caustic chemicals in eyes

A

eyes washed at eye was station for 15 min and seek medical attention

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29
Q

name 5 safety features all clinical labs should have

A

fire blanket, PPE, eye wash station, fire extinguisher, first aid kit

30
Q

T.C.

A

to contain. contents of pipette should be rinsed out with the solution it is being added to

31
Q

T.D

A

to deliver. contents of pipette should be blown out

32
Q

controls the amount of light

A

aperture

33
Q

glassware resists normal cleaning should be cleaned with what?

A

potassium sodium dichromate and concentrated sulfuric acid

34
Q

name two brand names of heat resistant glassware

A

pyrex and kimax

35
Q

bulb in the center calibrated to deliver a fixed volume of liquid used when great accuracy is needed.

A

volumetric or transfer pipettes

36
Q

graduated pipette delivers full volume mark to mark

A

serological pipettes

37
Q

pipette with bulb close to delivery tip used for viscous liquids

A

ostawald-folin pipette

38
Q

delivers full volume mark to tip

A

graduated or measuring pipette

39
Q

what does an etched ring near the mouth of the pipette mean

A

blow out pipette

40
Q

flat bottom and sides that slpe up to the neck. used for noncritical volumes. used for mixing and preparing reagents.

A

erlenmeyer flask

41
Q

wide mouth straight sides spout for pouring. used for estimating liquid amounts for fixing, heating and storage

A

beaker

42
Q

cylindrical glass or plastic with several calibrated markings from 5mL to 2L. Not used for precise volume but maybe for reagent water.

A

graduated cylinders

43
Q

calibrated and used for more precise measurements

A

volumetric or florence flask

44
Q

what is low actinic glass used for?

A

light sensitive solutions

45
Q

name the most common material used for plasticware in the lab

A

polyethylene

46
Q

reference material from which 99.95% of the chemical can be retrieved

A

primary standard

47
Q

reference material in which the analyte concentration has ben ascertained by reference to a primary standard

A

secondary standard

48
Q

A.R.

A

analytical reagent high degree of purity most commonly used in clinical labs

49
Q

C.P.

A

chemically pure limit of impurities tolerated. suitable for general applications

50
Q

N.F.

A

national formulary

51
Q

U.S.P

A

united states pharmacopcia less pure than chemically pure. Used in medicines.

52
Q

boiled water, steam cooled, vapors collected minerals remain behind contain dissolved gasses and organic solvents

A

distilled water

53
Q

water passes through charged resin particles. the particles combine with ions present in water to remove them. removes substances carrying electrical charges.

A

deionized water

54
Q

redistilled water to get an even high degree of purity

A

double distilled water

55
Q

ideal for consituting chemistry and coag products preparing standards for rinsing electrodes. *reverse osmosis mixed bed deionizedation activated carbon filtration and final filtration and it should be for organic and inorganic particulant and soluble contanminents

A

reagent grade water

56
Q

proper storage of reagents and chemicals

A

cool dry area, properly labeled, and proper temperature

57
Q

5 things a reagent label must contain

A

date it was made

name of reagent

person who made it

cautions

expiration date

58
Q

what is the expiration date of a reagent if it is not specified?

A

1 month

59
Q

what steps must you take once you have transferred a chemical from the weigh boat into a receiving vessel to ensure an accurately prepared solution?

A

rinse weigh boat and funnel with solvent

60
Q

fixed angle rotor centrifuge

A

cups are positioned at a fixed angle

61
Q

horizontal rotor centrifuge

A

cups are in a vertical position when not operating and assume a horizontal position when operating

62
Q

can reach high speeds up to 10,000 rpms and its used to sperate specimens that take several hours or days

A

ultra centrifuge

63
Q

used most often in hematology to centrifuge small amounts of blood to determine hematocrit (HCT)

A

microhematocrit centrifuge

64
Q

table or floor model can spin large amounts of blood and is refrigerated

A

refrigerated blood band centrifuge

65
Q

small centrifuge (high or low) for spinning serum and or cell mixtures

A

serofuge

66
Q

used in blood bank to wash RBC’s

A

cell washers

67
Q

makes slides in a monolayer and is used for body fluid analysis

A

cytospin centrifuge

68
Q

list the precautions that should be taken when operating a centrifuge

A

*all glass breakage must be cleaned immediately as well as any spillage *never stop with hands *keeps caps on all viles or parafilm it *do not open lid till it has completely stopped

69
Q

define the accuracy of a trip balance vs. an analytical balance

A

trip accuracy is 0.1 g analytical accuracy 0.1mg

70
Q

describe the proper placement of an analytical balance

A

flat stable surface

away from heat or air current

away from high traffic or movement

71
Q

what function does the glass enclosure and the balancing screws have on an analytical balance?

A

glass enclosure protects from air currents balancing screws level the balance

72
Q

what is the proper way to handle objects that are to be weighed?

A

forceps or thongs

Intro to MLT 110 study guide 3 (3 decks)

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