study guide 3

Question 1

what removes dried oil from the lens the best?

Answer 1

Xylene

Question 2

solid cone of light delivered to the specimen by the condenser and gathered by the objective

Answer 2

mumerical aperture on the microscope

Question 3

ability of the scope to reveal or seperate fine detail

Answer 3

resolution

Question 4

the distance from the center of the objective to the top of the specimen also called the focal length

Answer 4

working distance

Question 5

imperfect refraction or focalization of a lens; inability to bring light rays to a single focus

Answer 5

abberation

Question 6

reflects the beam of light upward

Answer 6

mirror

Question 7

directs and focuses the light from the light source up to the specimen

Answer 7

condenser

Question 8

total magnification of a specimen on a microscope

Answer 8

ocular times objective

Question 9

what are the three most common objectives used in the clinical lab and what are they otherwise known as

Answer 9

10x-low 40-45x- high 100x-oil

Question 10

what magnification are most occulars

Answer 10

10x

Question 11

uses daylight or light bulb with a compound lens system

Answer 11

bright field microscope

Question 12

special condenser makes background appear dark while the specimen is light. The condenser allows light waves to cross on the specimen instead of through it.

Answer 12

dark field microscope

Question 13

equipped with a UV light source and special filter to allow shorter wave lenth.

Answer 13

flourescent microscopy

Question 14

special diaphragm into or below the condenser to view unstained structures

Answer 14

phase contrast microscope

Question 15

uses a special filter that takes ordinary light waves and allows only light waves of one orientation(north-south) to pass through the filter and reach the specimen. To illuminate objects that can rotate light.

Answer 15

polorized microscopy

Question 16

substitution of an electron bean for light rays to achieve greater resolution

Answer 16

electron microscope

Question 17

the light circle you see through the microscope

Answer 17

field of view

Question 18

what is a MSDS and what kind of information can a lab employee get from it?

Answer 18

material safety data sheet

describes hazards, safe handling, storage and disposal of hazardous chemicals

Question 19

what do the colors of the diamond hazard symbol give you information about?

Answer 19

red-fire hazard yellow-reactivity blue-health hazard white-specific hazard rate them 0-4

Question 20

treat all specimens as potentially infectious

Answer 20

standard precautions

Question 21

class A

Answer 21

anthrax, botulism, plague, small pox, tularemia, ebole

Question 22

class B

Answer 22

bacterial, C perfringens, ecoli 105H7, salmonella, shigella,

Question 23

Class C

Answer 23

emerging pathogens that could be engineered

Question 24

what is the single most effective way to reduce the possibility of contaminating yourself?

Answer 24

washing your hands

Question 25

How long is HBV stable in blood products at 25 degrees C

Answer 25

7 days

Question 26

where should contaminated materials be disposed?

Answer 26

bio hazard storage

Question 27

caustic chemicals on skin

Answer 27

skin washed off with water for 5 mins immediately after exposure.

Question 28

caustic chemicals in eyes

Answer 28

eyes washed at eye was station for 15 min and seek medical attention

Question 29

name 5 safety features all clinical labs should have

Answer 29

fire blanket, PPE, eye wash station, fire extinguisher, first aid kit

Question 30

T.C.

Answer 30

to contain. contents of pipette should be rinsed out with the solution it is being added to

Question 31

T.D

Answer 31

to deliver. contents of pipette should be blown out

Question 32

controls the amount of light

Answer 32

aperture

Question 33

glassware resists normal cleaning should be cleaned with what?

Answer 33

potassium sodium dichromate and concentrated sulfuric acid

Question 34

name two brand names of heat resistant glassware

Answer 34

pyrex and kimax

Question 35

bulb in the center calibrated to deliver a fixed volume of liquid used when great accuracy is needed.

Answer 35

volumetric or transfer pipettes

Question 36

graduated pipette delivers full volume mark to mark

Answer 36

serological pipettes

Question 37

pipette with bulb close to delivery tip used for viscous liquids

Answer 37

ostawald-folin pipette

Question 38

delivers full volume mark to tip

Answer 38

graduated or measuring pipette

Question 39

what does an etched ring near the mouth of the pipette mean

Answer 39

blow out pipette

Question 40

flat bottom and sides that slpe up to the neck. used for noncritical volumes. used for mixing and preparing reagents.

Answer 40

erlenmeyer flask

Question 41

wide mouth straight sides spout for pouring. used for estimating liquid amounts for fixing, heating and storage

Answer 41

beaker

Question 42

cylindrical glass or plastic with several calibrated markings from 5mL to 2L. Not used for precise volume but maybe for reagent water.

Answer 42

graduated cylinders

Question 43

calibrated and used for more precise measurements

Answer 43

volumetric or florence flask

Question 44

what is low actinic glass used for?

Answer 44

light sensitive solutions

Question 45

name the most common material used for plasticware in the lab

Answer 45

polyethylene

Question 46

reference material from which 99.95% of the chemical can be retrieved

Answer 46

primary standard

Question 47

reference material in which the analyte concentration has ben ascertained by reference to a primary standard

Answer 47

secondary standard

Question 48

A.R.

Answer 48

analytical reagent high degree of purity most commonly used in clinical labs

Question 49

C.P.

Answer 49

chemically pure limit of impurities tolerated. suitable for general applications

Question 50

N.F.

Answer 50

national formulary

Question 51

U.S.P

Answer 51

united states pharmacopcia less pure than chemically pure. Used in medicines.

Question 52

boiled water, steam cooled, vapors collected minerals remain behind contain dissolved gasses and organic solvents

Answer 52

distilled water

Question 53

water passes through charged resin particles. the particles combine with ions present in water to remove them. removes substances carrying electrical charges.

Answer 53

deionized water

Question 54

redistilled water to get an even high degree of purity

Answer 54

double distilled water

Question 55

ideal for consituting chemistry and coag products preparing standards for rinsing electrodes. \*reverse osmosis mixed bed deionizedation activated carbon filtration and final filtration and it should be for organic and inorganic particulant and soluble contanminents

Answer 55

reagent grade water

Question 56

proper storage of reagents and chemicals

Answer 56

cool dry area, properly labeled, and proper temperature

Question 57

5 things a reagent label must contain

Answer 57

date it was made name of reagent person who made it cautions expiration date

Question 58

what is the expiration date of a reagent if it is not specified?

Answer 58

1 month

Question 59

what steps must you take once you have transferred a chemical from the weigh boat into a receiving vessel to ensure an accurately prepared solution?

Answer 59

rinse weigh boat and funnel with solvent

Question 60

fixed angle rotor centrifuge

Answer 60

cups are positioned at a fixed angle

Question 61

horizontal rotor centrifuge

Answer 61

cups are in a vertical position when not operating and assume a horizontal position when operating

Question 62

can reach high speeds up to 10,000 rpms and its used to sperate specimens that take several hours or days

Answer 62

ultra centrifuge

Question 63

used most often in hematology to centrifuge small amounts of blood to determine hematocrit (HCT)

Answer 63

microhematocrit centrifuge

Question 64

table or floor model can spin large amounts of blood and is refrigerated

Answer 64

refrigerated blood band centrifuge

Question 65

small centrifuge (high or low) for spinning serum and or cell mixtures

Answer 65

serofuge

Question 66

used in blood bank to wash RBC's

Answer 66

cell washers

Question 67

makes slides in a monolayer and is used for body fluid analysis

Answer 67

cytospin centrifuge

Question 68

list the precautions that should be taken when operating a centrifuge

Answer 68

\*all glass breakage must be cleaned immediately as well as any spillage \*never stop with hands \*keeps caps on all viles or parafilm it \*do not open lid till it has completely stopped

Question 69

define the accuracy of a trip balance vs. an analytical balance

Answer 69

trip accuracy is 0.1 g analytical accuracy 0.1mg

Question 70

describe the proper placement of an analytical balance

Answer 70

flat stable surface away from heat or air current away from high traffic or movement

Question 71

what function does the glass enclosure and the balancing screws have on an analytical balance?

Answer 71

glass enclosure protects from air currents balancing screws level the balance

Question 72

what is the proper way to handle objects that are to be weighed?

Answer 72

forceps or thongs