Structure & Function of blood cells Flashcards
List steps involved with looking at blood cells in microscope
- Get some blood
- Don’t let it clot; Put it in a tube with EDTA in it
- Put a drop on a slide
- Use another slide to spread the blood out
- Let it dry
- Stain it with “wright-giemsa” stain
What chemical is in a test tube prior to putting blood in it? What is the purpose
EDTA, necessary to prevent blood from clotting in test tube
When examining blood under a microscope, where is the best location to view it on a slide?
Want to look at slide close to the edge of the smear (shown in oval below.) If you look too far out, cells look flat. Too far in, cells are all clumped together
![](https://s3.amazonaws.com/brainscape-prod/system/cm/135/084/641/a_image_thumb.png?1659453111)
List properties of Eosin. Describe what Eosin stain will bind to
Eosin is:
(aromatic/acidic), -charged, soluble in ethanol NOT water
Since it is -charged, it will bind to hydrophobic basic ( +charged) compounds, i.e. hemoglobin.
Eosinophil = “like eosin”
List properties of Methylene blue. Describe what methylene blue stain will bind to
Methylene blue is:
aromatic/basic, +charged, soluble in water or methanol
Since it is basic (+charged), it will bind to hydrophobic acidic macromoleclues (i.e. nucleic acids, some proteins)
Basophil = “like basic shit” i.e. basic bitches
Eosinophil count elevated in?
Allergic reactions
Parasitic infections
neoplastic processes (to be discussed later)