Structure and Function- General Flashcards
Midterm
Colony Morphology
Characteristics of the Colony produced by a species on solid agar
Examples of morphology
Size, Shape, Texture Colour
Morphotype
Colony with specific characteristics
Characteristics are influenced by:
Medium
Incubation conditions
Properties of all Cells (3)
Metabolism
Growth
Evolution
Properties of Some Cells (4)
Differentiation
Communication
Genetic Exchange
Motility
Which cell Property is the following:
Cells take up nutrients, transform them, and expels wastes
Metabolism
Which cell Property is the following: Nutrients from the environment atre converted into new materials to formnew cells
Growth
Which cell Property is the following:
Cells evolve to display new properties, Phylogenetic trees capture evolutionary relationships
Evolution
Which cell Property is the following:
Can form new cell structures such as a spore.
Differenciation
Which cell Property is the following:
Cells interact with each other by chemical messengers
Communication
Which cell Property is the following:
cells can exchange genes by several mechanisms
Genetic Exchange
Which cell Property is the following:
Are capable of Self-propulsion (Flagellum)
Motility
Two types of metabolism (Functions of cell)
Genetic
Catalytic
What is the genetic function of the cell? (Metabolism)
DNA Replication
DNA → Transcription → RNA → Translation → Proteins
What is the Catalytic Functions of the cell? (Metabolism)
Energy Conservation (ADP + Pi → ATP) Generation of precursors of macromolecules (Metabolism)
Enzyme (Metabolic Catalysts)
Intracellular Organelles of Prokaryotes
Cell Wall
Cytoplasmic Membrane
Nucleoid
Cytoplasm
Plasmid
Ribosomes
Prokaryotes are
Bacteria and Archea
Eukaryotes are
Eukarya
Intracellular Organelles of Eukaryotes
Cell Wall
Cytoplasmic Membrane
Mitochondrion
Nuclear Membrane
Nucleus
Ribosomes
Endoplasmic Reticulum (ER)
Cytoplasm
Golgi Complex
Three functions of the membrane
Permeability Barrier
Protein Anchor
Energy Conservation
What function fo the membrane is described:
Prevent leakage and a gateway for transport of nutrient into and waste out of cell
Permeability barrier
What function fo the membrane is described:
Site of many proteins that participate in transport, bioenergetics, and chemotaxis
Protein Anchor
What function fo the membrane is described:
Site of generation and use of the proton motive force
Energy conservation
Ratio Protein/Lipids:
50% or less
Types of membrane proteins
Sensors
Adhesins
Transporters
Enzymes
Type of membrane
Phospholipid Bilayer
Bacteria/Eukarya
Phospholipid bilayer linkage
Ester linkage
Archea Phospholipid Bilayer linkage
Ether linkage
The Archeal Lipid chain is composed of
Isoprene
Waht is a unique charactiristic in the archeal membrane and what is its use
Can be a lipid monolayer (Biphytanyl)
It is more resistant to extreme environments → More stable at high temperature
What are the three type of archeal membranes (bi/mono layer)
Glyceroldiether (Bi)
Diglycerol tetraethers (Mono)
Crenarchaeol (Mono)
How is the Lipid bilayer stabilized in Eukarya?
Sterol (Consist of Polar head, Rigid planar steroid ring structure, nonpolar hydrocarbon tail)
How is the Lipid bilayer stabilized in Animal Cells
Cholesterol
How is the Lipid bilayer stabilized in Fungi
Ergosterol (enzyme inhibitor for ergosterosis synthesis used to rid of yeast infections)
How is the Lipid bilayer stabilized in Plant cells and Protozoans
Stigmasterol
In bacteria and Archea Sterols are
Almost always absent
How is the membrane stabilized in Bacteria
Hopanoids
What are the limts of the size of a cell?
0.5 micrometers to 750 micrometers in diameter
What does the Surface volume ratio affect?
exchanges with the outside (Capacity to transport substrate across the cytoplasmic membrane)
What affetc the Surface volume ratio?
the Shape of the cell
What enzyme is in charge of DNA Replication?
DNA Polymerase
What enzyme is in charge of DNA transcription?
RNA Polymerase
What enzyme is in charge of RNA translation into protein?
Ribosome
The following characteristics correspond to Storage of DNA in:
Circular molecule, double stranded.
Bacteria/Archea
The following characteristics correspond to Storage of DNA in:
Generally haploid (one copy)
Bacteria/Archea
The following characteristics correspond to Storage of DNA in:
Packaged with proteins (H-NS and other Histone-like protein), aggregates to form the nucleoid), the chromosome
Bacteria/Archea
The following characteristics correspond to Storage of DNA in:
DNA in the cytoplasm, ribosomal RNA encoded on the chromosome
Bacteria/Archea
The following characteristics correspond to Storage of DNA in:
May also contain plasmids
Bacteria/Archea
The following characteristics correspond to Storage of DNA in:
Linear molecules, double-stranded.
Eukarya
The following characteristics correspond to Storage of DNA in:
Generally diploid (two copies)
Eukarya
The following characteristics correspond to Storage of DNA in:
Packaged with proteins (Histones) to form chromatin fibers, the chromosome
Eukarya
The following characteristics correspond to Storage of DNA in:
DNA in the nucleus
Eukarya
The following characteristics correspond to Storage of DNA in:
Nucleolus: (DNA that code for ribosomal RNA, ribosomal proteins, immature ribosome)
Eukarya
Ribosome Function
Composed of ribosomal RNA and proteins, translates mRNA into amino acid chains to form proteins.
Ribosome location in Prokaryotes
Free in the cytoplasm or attached to cytoplasmic membrane.
Ribosome location in Eukaryotes
Free in the cytoplasm or bound to the endoplasmic reticulum
Number of ribosomes and subunits in Prokaryotes
30S + 50S subunits = 70S ribosome
Number of ribosomes and subunits in Eukaryotes
40S + 60S subunits = 80S ribosome
Describes the rate of sedimentation of a particle in an ultracentrifuge. Proportional to the size, shape and density of the particle but the relationship is not linear
Svedberg unit