Strom-1 Flashcards
What tube will prevent your blood sample from clotting ?
EDTA tube with the purple top. It chetalates the Ca and prevents the clot from forming
What would you stain this cell with ?
Eosin which is acidic and aromatic and will bring out the color in RBC’s
What would you stain this cell with and what does it do and what is its name ?
This Granulocyte is an Eosinophil (You stain with eosin) - It plays a key role in killing antibody coated parasites by releasing its granule contents. Granules contain mediators of inflammation and toxic compounds.
**Notice the bilobed nucleus and granules.
What kind of cell is this and what would you stain it with ?
It is a basophil and you stain it with methyline blue. This granulocyte controlls the immune responses to parasites.
* they are related to tissue mast cells and are the least abundant cell in the immune system.
What is the cell on the left and what is the cell on the right ?
The cell on the left is a Monocyte and the cell on the right is a lymphocyte
What kind of cell is this ?
A Neutrophil. This is the cell of acute inflammation and is the most abundant in the body
They can degranulate, phagocytose, and even kill invaders after they are dead
Segmented Nucleus
Ya they are storm troopers
Do monocytes have granules ?
No
Where do monocytes become macrophages ?
In the peripheral organs
What are the lymphocytes ?
T cells
B Cells
NK Cells
How can you tell a reactive lymphocytes from a normal lymphocyte ?
It iwll have more cytoplasm and a more prominant nucleus
When evaluating a smear what do you multiply the platelet number by ?
What do you multiply WBC’s by ?
20,000
300
When RBC’s vary by size …
Anisocytosis
When RBC’s vary in shape ?
Poikilocytosis
How can you evaluate the Hb content of a RBC ?
Center should be 1/3 the size of the total diameter and there should be no clear demarcation between the outside and periphery.
What are the regenerative signs of RBC’s ?
Diffusely baosphillic cells stppled cells and nucleated cells.
What are these cells ?
Immature Neutrophils which are called bands.
Notice the non-segmented horseshoe shaped nucleus
The less mature bean shaped nucleus will be a metamyelocyte
How can you differentiate bands from monocytes ?
Monocytes will be larger with grey cytoplasm
What is strange about this neutrophil ?
Notice the toxic granulation in the cytoplasmic granules. This is indicative of an infection
What kind of cells is this and what is strange about it ?
This is a Reactive Lymphocyte Notice the Large Nuclei and abundant cytoplasm
What are the seven important aspects of the RBC that allow it to function properly
- Flexible Shape
- Durable membrane
- Maz Hb O2 capacity
- Offset Osmotic pressure
- Anti-Oxidant System
- Anaerobic energy supply
- Ability to avoid complement
What are two unique molecules that allow the RBC its flexile shape
Ankryn and Spectrin
What are the two aspects of the RBC anti-oxidant system ?
A way to reduce Methemoblobulin which requires NADH
Glutathionine Reductase which requires NADPH
How are NADPH and NADH made by the RBC ?
NADH is made by Anaerobic glycolysis
NADPH is made by the pentose phosphate shunt
What two molecules allow RBC’s to avoid complement ?
DAF and CD59
