STREPTOCOCCUS Flashcards
STREPTOCOCCUS CHARACTERISTICS
G (+) cocci in pairs/ in chains,
nonmotile
NSF
Catalase (-)
Facultative anaerobes
Oxidase (-)
growth is enhanced by blood, serum, or glucose
Lancefield Classification
Group A
Streptococcus pyogenes
Beta Hemolysis
Lancefield Classification
Group B
Streptococcus agalactiae
Beta Hemolysis
Lancefield Classification
Group C
S. equisimilis
S. zooepidimicus
S. equi
S. dysagalactiae
Beta Hemolysis
Lancefield Classification
Group D Enterococci
E. faecalis
E. faecium
E. durans
E. avium
Beta Hemolysis
Lancefield Classification
Group D Non Enterococci
S. bovis
S. equinis
Beta Hemolysis
Alpha Hemolytic Reaction
S. pneumoniae
Alpha and Gamma Hemolytic Reaction
Virdans
Streptococci
- Group A
- Beta hemolysis pattern
- Pathogenic to man acquired through
droplets - flesh-eating bacteria
STREPTOCOCCUS PYOGENES
VIRULENCE FACTORS OF S. pyogenes
M protein
Protein F
Lipoteichoic Acid
ENZYMES AND TOXINS THAT ARE
PRODUCED by S. pyogenes
Hemolysin
Streptolysin O
Streptolysin S
Deoxyribonuclease
Streptokinase
Hyaluronidase
Pyrogenic Toxins
CLINICAL INFECTION of S. pyogenes
Pharyngitis/Strep throat
Impetigo
Scarlet fever
Cellulitis
Erysipelas
Necrotizing fasciitis
Streptococcal Toxic Shock Syndrome
- Group B
- Beta hemolytic pattern
- Normal flora in the female genital urinary
tract. - Nosocomial transmitted but infects the
fetus and infants
STREPTOCOCCUS AGALACTIAE
VIRULENCE FACTORS OF S. agalactiae
Capsule
Hemolysin
CAMP factor
Neuraminidase
Dnase
Hyaluronidase
Hyaluronidase
Protease
CLINICAL INFECTION of S. agalactiae
Pneumonia
Meningitis
Sepsis
Postpartum Infection
Osteomyelitis
UTI
Endocarditis
- Beta hemolytic pattern
- Animal pathogen
- Have Streptokinase enzyme
GROUP C STREPTOCOCCI
GROUP D STREPTOCOCCI
CLINICAL INFECTIONS
Wound infection
Endocarditis
UTI
Sepsis
These are oropharyngeal commensals
S. mutans group
Salivarius
S. bovis
S. constellatus
dental caries and sub-acute endocarditis
S. mutans
- Pneumococcus
- Lancet or oval bullet shape
- Common isolate both as a pathogen and
as a member of normal flora.
STREPTOCOCCUS PNEUMONIAE
- Normal flora and pathogens in the
respiratory tract. - Colonies Appearance: Dome Shape
- In other book: Cone with ice cream
STREPTOCOCCUS PNEUMONIAE
CLINICAL INFECTIONS IN STREPTOCOCCUS PNEUMONIAE
Bacterial lobarpneumonia
Bacteria meningitis in adults
Otitis media
Bacteremia
Endocarditis
VIRULENCE FACTORS OF STREPTOCOCCUS PNEUMONIAE
Capsular
Polysaccharide
Hemolysin
Immunoglobulin A
Protease
Neuraminidase
Hyaluronidase
BACITRACIN SUSCEPTIBILITY TEST RESISTANT
Group B. (S. agalactiae)
BACITRACIN SUSCEPTIBILITY TEST SENSTIVE
Group A. (S. pyogenes)
SULFAMETHOXAZOLETRIMETHROPRIM SUSCEPTIBILITY TEST RESISTANT
Group A. (S. pyogenes)
SULFAMETHOXAZOLETRIMETHROPRIM SUSCEPTIBILITY TEST SENSTIVE
Group B. (S. agalactiae)
The arrowhead hemolytic pattern results when
the organism is streak perpendicular to betahemolytic staphylococcus aureus.
CHRISTIE-ATKINS-MUNCH-PETERSON
(CAMP TEST)
POSITIVE FOR CAMP TEST
“ARROWHEAD
HEMOLYSIS”
Group B. (S. agalactiae)
NEGATIVE FOR CAMP TEST
NO ZONE OF HEMOLYSIS
Group A. (S. pyogenes)
Hipurric acid is converted to glycine and benzoic
acid in the presence of enzyme hippuricase.
Ninhydrin added to glycine produces purple
color
The end product of the hippurate-hydrolysis test is purple color/deep purple color.
HIPPURATE HYDROLYSIS TEST
POSITIVE FOR HIPPURATE HYDROLYSIS TEST
DEEP PURPLE COLOR
B. (S. agalactiae)
NEGATIVE FOR HIPPURATE HYDROLYSIS TEST
YELLOW PINK
OR COLORLESS
A. (S. pyogenes)
RESISTANT IN OPTOCHIN (TAXO P)
VIRIDANS STREPTOCOCCI
SENSITIVE IN OPTOCHIN TAXO P
PNEUMOCOCCI
2 KINDS of OPTOCHIN
- 10 microgram disks
- 6 microgram disks
more concentration of
antibiotics
- Zone of inhibition: at least 14 mm
10 microgram disks
- Zone of inhibition: 10 mm
6 microgram disks
Greater 14 ml zone on inhibition
SUSCEPTIBLE/SENSITIVE
(STREPTOCOCCUS PNEUMONIA)
SUSCEPTIBLE/SENSITIVE
(STREPTOCOCCUS PNEUMONIA)
RESISTANT (VIRIDANS STREPTOCOCCI)
For capsular swelling
S. pneumoniae has capsule- POSITIVE
NUEFELD- QUELLUNG
Salt Tolerance Test
(Growth In 6.5%) POSITIVE
GROUP D ENTEROCOCCUS
Salt Tolerance Test
(Growth In 6.5%) NEGATIVE
GROUP D NON ENTEROCOCCUS
Penicillin Susceptibility Test RESISTANT
GROUP D ENTEROCOCCUS
Penicillin Susceptibility Test SUSCEPTIBLE
GROUP D NON ENTEROCOCCUS
Pyrrolidonyl Arylamidase (PYR) POSITIVE
GROUP D ENTEROCOCCUS
Pyrrolidonyl Arylamidase (PYR) NEGATIVE
GROUP D NON ENTEROCOCCUS