MICROCOCCACEAE Flashcards
It includes Staphylococcus, Micrococcus,
Planococcus, Stomatococcus
Micrococcaceae
GENERAL CHARACTERISTICS OF MICROCOCCACEAE
catalase positive
non-motile
glucose fermenters
Modified oxidase test POSITIVE
MICROCOCCI
Modified oxidase test NEGATIVE
STAPHYLOCOCCI
Anaerobic acid production from glucose
POSITIVE
STAPHYLOCOCCI
Anaerobic acid production from glucose NEGATIVE
MICROCOCCI
Growth on Furoxone–Tween 80–oil red O agar POSITIVE
MICROCOCCI
Growth on Furoxone–Tween 80–oil red O agar NEGATIVE
STAPHYLOCOCCI
Anaerobic acid production from glycerol
in the presence of erythromycin POSITIVE
STAPHYLOCOCCI
Anaerobic acid production from glycerol
in the presence of erythromycin NEGATIVE
MICROCOCCI
Resistance to bacitracin
(0.04 units) RESISTANT
STAPHYLOCOCCI
Resistance to bacitracin
(0.04 units) SUSCEPTIBLE
MICROCOCCI
Lysosome
(50-mg disk) RESISTANT
STAPHYLOCOCCI
Lysosome
(50-mg disk) SUSCEPTIBLE
MICROCOCCI
Lysostaphin test RESISTANT
MICROCOCCI
Lysostaphin test SUSCEPTIBLE
STAPHYLOCOCCI
Under the microscope with gram staining
reaction: they are gram-positive cocci in
clusters or grape-like.
STAPHYLOCOCCI
GROWTH ON BAP: produces creamy
white, pin head colonies, and betahemolytic pattern.
STAPHYLOCOCCI
Gram-positive cocci in tetrads or sars
made.
MICROCOCCI
GROWTH ON BAP: yellow colonies and
nonhemolytic pattern (gamma).
MICROCOCCI
Uses 0.04 units of
bacitracin and performed
on BAP or MHA
BACITRACIN
BACITRACIN RESISTANT
STAPHYLOCOCCI
BACITRACIN SUSCEPTIBLE
MICROCOCCI
Uses 100 ug furazolidone
and is performed on BAP
FURAZOLIDONE SUSEPTIBILITY TEST
another antibiotic we
used to differ micrococci and
staphylococc
FURAZOLIDONE
FURAZOLIDONE RESISTANT
MICROCOCCI
FURAZOLIDONE SUSCEPTIBLE
STAPHYLOCOCCI
Rapid test
Test that detects
oxidase enzyme
MICRODASE TEST
Paper reagent
Tetramethyl-p -
phenylenediamine in
dimethyl sulfoxide
Microdase positive reaction and what organism is positive in this test?
blue to purple blue
MICROCOCCI
Microdase negative reaction and what organism is negative in this test?
No color change
STAPHYLOCOCCI
Under and anaerobic condition, staphylococci
ferment the glucose while micrococci fail to
produce acid
Micrococci will not ferment, they will just
oxidase
OXIDATION-FERMENTATION REACTION
