Strep

Question 1

Who am I? Aerobic G+ cocci, catalase (-)?

Answer 1

Strep sp.

Question 2

You have a G+ aerobic, catalase (-), beta hemolytic cocci - what test is next?

Answer 2

Bacitracin sensitivity OR PYR testing.

Question 3

Who am I? Aerobic G+ cocci, catalase (-), beta hemolytic, PYR (+) and/or bacitracin sensitive?

Answer 3

Strep pyogenes

Question 4

You have a G+ aerobic, catalase (-), beta hemolytic cocci that is PYR (-) and/or bacitracin sensitive - what test is next?

Answer 4

Hippurate hydrolysis or CAMP test

Question 5

This test detects organisms that take hippuric acid and hydrolyzed it to benzoic acid and glycine by the enzymatic action of hippuricase.

Answer 5

Hippurate hydrolysis test

Question 6

The basis of the __ test is the enhanced hemolytic activity of beta-hemolysin-producing strains of Staph aureus by an extracellular protein ( __ factor) produced by group B Strep (i.e., it distinguishes between beta hemolytic Staph aureus and group B strep).

Answer 6

CAMP; CAMP

Question 7

Who am I? Aerobic G+ cocci, catalase (-), beta hemolytic, PYR (-) and/or bacitracin resistant, hippurate hydrolysis and/or CAMP positive?

Answer 7

Strep agalactiae

Question 8

Who am I? Aerobic G+ cocci, catalase (-), beta hemolytic, PYR (-) and/or bacitracin resistant, hippurate hydrolysis and/or CAMP negative?

Answer 8

Beta hemolytic strep non-group A or B.

Question 9

What sp. is group A strep? Group B?

Answer 9

S. pyogenes

S. agalactiae

Question 10

You have a G+ aerobic, catalase (-), alpha hemolytic cocci - what test is next?

Answer 10

Optichin sensitivity.

Question 11

Optochin is a quinine derivative that helps to identify __ __ due to its sensitivity.

Answer 11

Strep pneumoniae

Question 12

You have a G+ aerobic, catalase (-), alpha hemolytic cocci that is optichin resistant - what test is next?

Answer 12

bile esculine

Question 13

How does the bile esculin test work?

What does a positive test look like?

Answer 13

Gram-positive bacteria other than some strep and enterococci are inhibited by the bile salts.

Dark brown or blackened.

Question 14

Who am I? You have a G+ aerobic, catalase (-), alpha hemolytic cocci that is optichin resistant and doesn’t grow in bile esculin media?

Answer 14

Viridans strep

Question 15

You have a G+ aerobic, catalase (-), alpha hemolytic cocci that is optichin resistant and grows in bile esculin media - what test is next?

Answer 15

PYR in 6.5% NaCL

Question 16

Who am I? You have a G+ aerobic, catalase (-), alpha hemolytic cocci that is optichin resistant, bile esculin media (+), and PYR 6.5% NaCl (+)?

Answer 16

Group D enterococcus

Question 17

Who am I? You have a G+ aerobic, catalase (-), alpha hemolytic cocci that is optichin resistant, bile esculin media (+), and PYR 6.5% NaCl (-)?

Answer 17

Group D non-enterococcus

Question 18

You have a G+ aerobic, catalase (-), gamma hemolytic cocci - what test is next?

Answer 18

Bile esculin

Question 19

Who am I? You have a G+ aerobic, catalase (-), gamma hemolytic cocci that doesn’t grow in bile esculin?

Answer 19

non-hemolytic strep

Question 20

You have a G+ aerobic, catalase (-), gamma hemolytic cocci that grows in bile esculin - what test is next?

Answer 20

PYR 6.5% NaCl

Question 21

Who am I? You have a G+ aerobic, catalase (-), gamma hemolytic cocci that grows in bile esculin and is PYR 6.5% NaCl positive?

Answer 21

Group D enterococcus

Question 22

Who am I? You have a G+ aerobic, catalase (-), gamma hemolytic cocci that grows in bile esculin and is PYR 6.5% NaCl negative?

Answer 22

Group D non-enterococcus

Question 23

Is group D non-enterococcus alpha, beta, or gamma hemolytic?

Answer 23

It can be either alpha or gamma hemolytic