Staph Flashcards

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1
Q

The first test to run on cocci grown aerobically?

What test should follow?

A

Gram’s stain.

Catalase.

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2
Q

What is catalase?

What does a positive catalase test look like?

A

An enzyme (produced by aerobes) used to neutralize oxidative toxins (such as H2O2).

Bubbles

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3
Q

You have a G+ aerobic, catalase + cocci - what test is next?

A

coagulase

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4
Q

What methods can be used to test the presence of coagulase?

A

Slide (agglutination) or tube (clot).

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5
Q

Who am I? G+ aerobic clusters, catalase +, coagulase+

A

S. aureus

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6
Q

You have a G+ aerobic cocci, catalase (+), coagulase (-) - what test is next?

A

Bacitracin sensitivity

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7
Q

Who am I? Aerobic G+ cocci, catalase (+), coagulase (-), bacitracin (+/sensitive).

A

Micrococcus sp.

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8
Q

What is the PYR test (L-Pyrrolidonyl-β-naphthylamide)?

A

Test used to detect an enzyme that breaks down specific protein structures.

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9
Q

Who am I? Aerobic G+ cocci, catalase (+), coagulase (-), bacitracin (-/resistant), PYR (+)?

A

Staph. lugdenesis

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10
Q

Aerobic G+ cocci, catalase (+), coagulase (-), bacitracin (-/resistant), PYR (-) - what’s the next test?

A

novobiocin resistance

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11
Q

Aerobic G+ cocci, catalase (+), coagulase (-), bacitracin (-/resistant), PYR (-), novibiocin (+/sensitive)?

Aerobic G+ cocci, catalase (+), coagulase (-), bacitracin (-/resistant), PYR (-), novibiocin (-/resistant)?

A

Staph. epi

Staph. saprophyticus

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12
Q

What test distinguishes staph from strep?

A

catalase

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