Strain Improvement Flashcards
what are the phases of metabolism
tropophase- growth in nutrient excess and idiophase- nutrient limitation
what is the role of mutation
genes can be mutated through random mutation or DNA recombination and then screening occurs for desired products
what types of mutagens are there
random mutation, radiation, chemical and biological
explain fungal evolutionary engineering
they couldn’t use 5C sugars until genes were introduced (metabolic engineering) and the fungus produced was screened (evolutionary engineering)
what is the role of gene cloning
it can help identify function. extra copies can be added or deletion by homologous recombination with a selectable marker
what is transformation
the uptake of genes by other organisms- usually bacteria
how does transformation occur
Ca2+ in gram -ve bacteria, protoplasts or polyethene glycol in gram +ve bacteria (similar in fungi)
who does ACNFP advise
FSA (food standards agency), DEFRA (UK) and the department of health
when should GM products be labelled
when they are ingredients active in the product or products of a GM source. Animals fed on GM feed and processing aids not functional in the final product don’t
