Sterilization and Disinfectant

Question 1

Total inactivation of all forms of microbial life

Answer 1

Sterilization

Question 2

Most resistant to sterilization

Answer 2

Spores

Question 3

Least resistant to sterilization

Answer 3

Viruses

Question 4

Destruction of microorganism capable of causing infection by means of germicidal agent

Answer 4

Disinfection

Question 5

T/F: Disinfectants are not necessarily sporicidal but they are sporostatic

Answer 5

True

Question 6

Agent that kills a microorganism

Answer 6

Germicide

Question 7

Germicide which inhibits the growth of bacteria

Answer 7

Bacteriostatic

Question 8

Germicide that kills bacteria

Answer 8

Bactericidal

Question 9

Topical chemical agent applied to a body surface or living tissue that kills or inhibits the growth of pathogenic microorganisms

Answer 9

Antiseptic

Question 10

Use of disinfectant to lower microbial content of inanimate objects

Answer 10

Sanitization

Question 11

T/F: Antiseptic is bactericidal in action

Answer 11

False

Question 12

T/F: Antiseptic is used at lowest concentration that results to desired effect

Answer 12

True

Question 13

Prevention of multiplication of microorganisms in formulated objects

Answer 13

Preservation

Question 14

Most reliable method of sterilization

Answer 14

Heat

Question 15

T/F: Dry heat is preferred over moist heat for sterilization

Answer 15

False

Question 16

Heat kills microorganisms by?

Answer 16

Protein denaturation

Question 17

Most efficient form of sterilization by heat

Answer 17

Autoclaving

Question 18

T/F: Boiling kills all vegetative forms of pathogenic organisms but not bacterial endospores

Answer 18

True

Question 19

Used for sterilizing materials that would be damaged by autoclaving

Answer 19

Fractional sterilization or tyndallization

Question 20

T/F: Relatively heat-resistant bacteria survive pasteurization and are likely to cause disease or cause refrigerated pasteurized products to spoil

Answer 20

False

Question 21

Dry heat kills microorganisms by?

Answer 21

Oxidation effects

Question 22

Most widely used form of dry heat

Answer 22

Hot air oven

Question 23

Form of dry heat used in decontamination of waste items prior to disposal

Answer 23

Incineration

Question 24

Form of dry heat used in inoculating loops and needles

Answer 24

Red heat flame

Question 25

T/F: Bacterial spores are resistant to drying or dessication

Answer 25

True

Question 26

Inhibiting bacterial metabolism and growth by exposing it to very low temperatures

Answer 26

Freezing and lyophilization

Question 27

T/F: Freezing destroys microorganisms

Answer 27

False

Question 28

Most effective bactericidal

Answer 28

Ultraviolet light

Question 29

T/F: Ionizing radiation has greater penetrating ability than UVL

Answer 29

True

Question 30

Cationic compounds are most effective at?

Answer 30

Alkaline pH

Question 31

T/F: Cationic compounds have an effect on lipid-enveloped viruses

Answer 31

False

Question 32

Disrupts lipoprotein framework of the CM; most effective at acid pH

Answer 32

Anionic compounds

Question 33

Causes leakage of cell contents and irreversible inactivation of membrane bound oxidases and dehydrogenases

Answer 33

Phenolic compounds

Question 34

T/F: Alcohols are sporicidal

Answer 34

False

Question 35

Acids and alkalis are for

Answer 35

Protein denaturation

Question 36

Exert their action through free H and OH ions and alter the pH of the organism’s environment

Answer 36

Acids and alkalis

Question 37

Inactivates enzymes converting functional SH groups to oxidized S-S for stronger agents

Answer 37

Oxidizing agents

Question 38

Oxidizing agents that destroy the cellular activity of proteins

Answer 38

Halogens

Question 39

Best antiseptic

Answer 39

Iodine