Sterilisation (IC5) Flashcards
What are 2 ways to test for sterility
1) By membrane filtration
Filter using 0.45 micron membrane, culture in growth medium
2) Direct inoculation
Remove sample aseptically, expose to growth medium, culture and note for bacteria growth
What is the fastest way to estimate amount of bacteria in filtration sterility testing
Most Probable Number (MPN)
Dilute broth 10 fold, count CFU and multiply to estimate original amount of bacteria
How to test sterility of Medical devices?
Direct Transfer sterility testing
Device must be in direct contact with Test Media
Any microbe on the device will grow and proliferate
Lumen is flushed with rinsing fluid → Filtered and placed in suitable media for incubation
How to prevent biofilm on vascular devices
Silver Sulfadiazine, on inner lining of endoscopy tube
How to prevent Healthcare Associated Infections? (4 points)
Limit transmission between patients and healthcare workers through adequate handwashing, glove use
Use appropriate prophylactic antimicrobials
Minimise invasive procedures
Surveillance of infections
Pharmacists role in HAI (6 points)
Store and distribute pharmaceutical preparations aseptically, limit potential transmission of infectious agents to patients
Maintain records of antibiotics distributed to medical departments
Provide summary reports and trends of antimicrobial use
Have information about antibiotics, toxic properties, substances that are incompatible with antibiotics
Develop guidelines for antiseptics, disinfectants
Quality control of techniques used to sterilise equipment
Terminal Sterilisation VS Aseptic Manufacturing
Terminal Sterilisation
Using lethal treatment on microbes eg. heat, radiation, chemical
One step process
Easy to reproduce and validate
Not suited for some materials
Aseptic manufacturing
Higher risk of contamination
Involves assessing the sterility of each step
More suitable for most materials
