Stem cells Flashcards

1
Q

what are stem cells

A

undifferentiated cells which can keep dividing to give rise to other cell types

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2
Q

definition of multipotent cells

A

are differentiated cells that can specialise into many different types of cells

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3
Q

definition of pluripotent cells

A

can give rise to many types of specialised cells, but not all cell types (such as placental cells)

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4
Q

definition of totipotent cells

A

undifferentiated cells that can divide and give rise to any type of specialised cells

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5
Q

what is a blastocyst

A

-a hollow ball of 50 or so cells
-a type of pluripotent stem cell
-the outer blastocyst cell layer goes on to form the placenta
-the inner 50 cells form the tissues of the developing embryo

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6
Q

definition of explant, callus and plantlet

A

explant- small piece of plant tissue transferred to growth medium

callus-a mass of undifferentiated cells

plantlet-young genetically identical plants

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7
Q

how to do tissue culture of plants

A

explants are taken from plants
-they are sterilised
-then placed on an agar medium with nutrients and growth hormones
-the cells divide to form a mass of undifferentiated cells called a callus
-the embryos develop into plantlets that are gentically identical clones

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8
Q

embryonic stem cells- where? type? use?

A

found in early stage embryos

pluripotent-can develop into almost any cell type in the body

uses- Due to their ability to differentiate into almost any cell type, embryonic stem cells have huge potential in the therapeutic treatment of many diseases

use is ethically debated

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9
Q

adult stem cells - where? type? use? risks?

A

found in bone marrow and brain

multipotent-can differentiate into a limited range of cell types

use- repair and maintaining tissues

risks- there is a chance that the cells used are rejected by the patient’s immune system if donor stem cells used

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10
Q

induced pluripotent stem cells

A

created by reprogramming adult cells into a pluripotent state

functions like embryonic stem cells w/o ethical concerns

useful for disease modelling and dry testing

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11
Q

therapeutically cloned stem cells

A

created using somatic cell nuclear transfer

can generate genetically matched stem cells for patients

used to repair damaged tissues

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12
Q

advantages and disadvantages of embryonic stem cells

A

+ can become any cell type

  • ethical concerns, risk of immune rejection
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13
Q

advantages and disadvantages of tissue/adult stem cells

A

+ no ethical concerns and taken from the patient’s own body

-limited differentiation ability, harder to obtain

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14
Q

risks of using stem cells

A
  • stem cells cultured in a lab could become infected with a virus
  • low numbers of stem cell donors
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15
Q

what did the acetabularia experiment find

A

The nucleus controls cell development using mRNA as a messenger molecule

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16
Q

what did the dolly sheep experiment find

A

All the genetic info for making a complete organism is present in every cell( except haploid and red blood cells)

17
Q

what did david and sargent find

A

As cells specialise, more genes are active and more mRNA is produced.

18
Q

How does differential gene expression happen?