Statistical Tests Flashcards

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1
Q

Purpose of t-test.

A
  • To compare 2 sets of mean
  • If they are significantly different or not
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2
Q

When is t-test used?

A

Data are:
- continuous
- normally distributed

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3
Q

Formula of t-test.

A
  • t = |Ā - B| / Square root of (SA^2/nA + SB^2/nB)
  • degrees of freedom = (nA - 1) + (nB - 1) = nA + nB - 2
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4
Q

Steps for t-test.

A
  1. let Ho: Ā = B
  2. Calculate s. d. of each sample.
  3. Calculate t-value.
  4. Find degrees of freedom.
  5. Find probability of Ā = B
  6. When P <= 0.05, Ā does not = B
    When P > 0.05, Ā = B
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5
Q

How to conclude for t-test?

A
  1. critical value (at degrees of freedom = n & p = 0.05) = x
  2. calculated t-value is, greater / lesser, than critical (t-) value
  3. null hypothesis, accepted / rejected (at p = 0.05)
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6
Q

Purpose of chi-squared test.

A
  • To test the goodness of fit between
  • O & E (observed & expected values)
  • When analysing results from:
  • genetics / breeding experiments
  • ecological sampling
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7
Q

When is chi-squared test used?

A

Data are:
- discrete
- categoric

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8
Q

Formula of chi-squared test.

A

χ^2= ∑(O-E)^2/E

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9
Q

Steps for chi-squared test.

A
  1. Set up null hypothesis, Ho: there is no significant difference between O & E.
  2. Calculate chi-squared value.
  3. Calculate degrees of freedom = n-1 (n = no. of classes)
  4. Use a chi-squared table to find P(χ2)
  5. If P(χ2) > 0.05:
    - chances of goodness of fit > 5%
    - accept Ho
    - O = E
  6. If P(χ2) <= 0.05:
    - chances of goodness of fit <= 5%
    - reject Ho
    - O P(χ2) > 0.05:
    - chances of goodness of fit > 5%
    - accept Ho
    - O ≠ E
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10
Q

How to conclude for chi-squared test?

A
  • Conclusion is made with 95% confidence.
  • (Because) the rejected value could be rejected wrongly
  • Allow 5% in conclusion
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11
Q

Purpose of Pearson’s linear correlation.

A

Determines whether there is linear correlation between 2 variables

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12
Q

When is Pearson’s linear correlation used?

A

Data must be:
- quantitative
- show normal distribution

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13
Q

Formula of Pearson’s linear correlation.

A

r = [ ∑ xy - nx̄ȳ ] / (n-1) SxSy

  • r = correlation coefficient
  • x = no. of species A
  • y = no. of species B
  • n = no. of readings
  • Sx = standard deviation of species A
  • Sy = standard deviation of species B
  • x̄ = mean no. of species A
  • ȳ = mean no. of species B
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14
Q

Steps for Pearson’s linear correlation.

A
  1. Create a scatter graph of data & identify if a linear correlation exists.
  2. State a null hypothesis, Ho = there is no correlation between the abundance of species A & species B.
  3. Use the following equation to work out Pearson’s correlation coefficient, r
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15
Q

How to conclude for Pearson’s linear correlation?

A

If the correlation coefficient, r is close to 1 or -1, then it can be stated that there is a strong linear correlation between the 2 variables & the null hypothesis can be rejected.

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16
Q

Purpose of Spearman’s Rank Correlation.

A

Determines whether there is correlation between variables that don’t show a normal distribution.

17
Q

When is Spearman’s Rank Correlation used?

A
  • There is an apparent relationship between 2 variables but
  • Data does not show a normal distribution
  • Data can be ranked
18
Q

Formula for Spearman’s Rank Correlation.

A

rs = 1 - (6 x ∑D^2 / n^3 - n)

  • rs = spearman’s rank coefficient
  • D = difference in rank
  • n = number of samples
19
Q

Steps for Spearman’s Rank Correlation.

A
  1. Create a scatter graph & identify possible linear correlation.
  2. State a null hypothesis, Ho = there is no correlation between the abundance of species A & species B.
  3. Use the Spearman’s Rank Correlation formula to work out the coefficient r.
  4. Refer to a table that relates critical values of rs to levels of probability.
20
Q

How to conclude for Spearman’s Rank Correlation?

A

If the value calculated for Spearman’s rank is greater than the critical value for the number of samples in the data (n) at the 0.05 probability level (p), then the Ho can be rejected, meaning there is a (positive / negative) correlation between 2 variables.

21
Q

Purpose of Simpson’s Index of diversity (D).

A

To calculate species diversity & evenness of abundance across different species in an area.

22
Q

When is Simpson’s Index used?

A

When data for species abundance has been calculated

23
Q

Formula for Simpson’s Index of Diversity.

A

d = 1 - (∑ (n / N)^2)

  • n = number of individuals
  • N = total number of organisms
24
Q

Steps for calculating Simpson’s Index of Diversity.

A
  1. Calculate (n / N) for each species
  2. Square each of these values
  3. Add them together & subtract the total from 1.
25
Q

How to conclude for Simpson’s Index of Diversity?

A
  • The value of D can fall between 0 and 1.
  • Value near 1 = high levels of biodiversity
  • Value near 0 = low levels of biodiversity
26
Q

Purpose of Lincoln’s Index.

A

Calculate an estimate of population size

27
Q

Formula for Lincoln’s Index.

A

N = n1 x n2 / m2

  • N = population estimate
  • n1 = number of marked individuals released from 1st sample
  • n2 = number of individuals in 2nd sample (marked & unmarked)
  • m2 = number of marked individuals in the 2nd sample
28
Q

State what standard deviation shows.

A
  • Spread of data from the mean value
  • Larger value = less reliable results