Staphylococcus Lab

Question 1

what are the 3 organisms in this experiment?

Answer 1

S. epidermidis
S. Saprophiticus
S. aureus

Question 2

what are the 3 types of hemolysis?

Answer 2

alpha, beta, gamma

Question 3

alpha

Answer 3

parti=

Question 4

beta

Answer 4

complete RBC breakdown; produces a clear zone around the urea of inoculation

Question 5

gamma

Answer 5

no hemolysis

Question 6

staphylococcic are halophilic meaning they are

Answer 6

salt loving and grown on MSA

Question 7

What are the 3 orgainsms on the MSA plate for the staphyloccocus test?

Answer 7

S. epidermidis
S. aureus
S. saprophyticus

Question 8

what does the result for the MSA plate look like ?

Answer 8

S. epidermidis grew meaninig its halophilic along with S. aureus. S. aureus is also a mannitol fermenter therefore, turns yellow on the plate

Question 9

what does the blood plate look like

Answer 9

burgundy-ish

Question 10

describe the coagulase test ?

Answer 10

the test tubes contain blood plasma and if the organism solidifies in the plasma then the coagulase test is positive. if the plasma remains liquid then the test is negative.

Question 11

what can grow on on EMB?

Answer 11

gram negative rods like e.coli

Question 12

what does EMB stand for ?

Answer 12

Eosin Methylene Blue agar

Question 13

when EMB turns that green sheen colot what has been fermented ?

Answer 13

lactose

Question 14

what is novobiosin ?

Answer 14

an anti-biotic

Question 15

what happened when novobiosin was placed in the middle of S. Saprophyticus, S. epidermidis, and S. aureus?

Answer 15

S. aureus and s. epidermidis were sensitive to the novobiosin and S. saprophyticus was resistant

Question 16

What was the MSA result for the nose, arm and fingernail?

Answer 16

The fingernail portion grew and was yellow meaning its a fermenter of mannitol and is halophillic.

Question 17

What was the Blood plate result for the nose, arm and fingernail?

Answer 17

arm= gamma hemolysis
nose= alpha
fingernail= beta hemolysis