Staphylococcus Flashcards
Staphylococci
Gram Positive cocci
Occur in singles, pairs, tetrads, short chains, but most commonly as irregular clusters
Suppurative (Pus-forming) infections
Members of the group Pyogenic Cocci
More than 50 species and subspecies
Most common is S. aureus
Catalase Test
Production of oxygen form hydrogen peroxide
Is used to distinguish Staphylococcus (Positive) from Streptococcus (negative)
Pathogenic Species
S. aureus
S. pseudintermedias
S. hyicus
Double Zone Hemolysis
On blood agar S. aureus and S. pseudintermedius produce two zones of hemolysis.
A narrow zone of complete hemolysis (a-hemolysis)
Wider zone of partial or incomplete hemolysis (B-hemolysis)
Staphylococcus aureus
“golden yellow” colored collonies
Grows well in medium containing 7.5-10% NaCl
Mannitol Salt agar is routinely used to selectively isolate S. aureus and distinguish from S. epidermidis.
Opportunistic and nosocomial pathogen.
Causes enteritis (Food poisoning; intoxication)
MRSA
Methicillin resistant S. aureus
Major clinical and epidemiological problem in human hospitals.
Also present in animals and are of zoonotic significance
Habitat
Occur in commensals of the skin of humans and animals.
Rugged cell wall allows them to withstand environmental insults such as drying, heat, high concentrations of salts.
Virulence Factors
Most of them have been studied.
Cell-Associated Components,
Exoenzymes and Exotoxins
Virulence Factors:
Cell-Associated Components:
Protein A
Surface protein that binds to Fc region of IgG molecules, disrupts opsonization and phagocytosis
Virulence Factors:
Cell-Associated Components:
Capsular Polysaccharides
11 serologically distinct capsule types in S, aureus of Humans and Cattle
Interfere with host defense mechanisms by inhibiting attachement of antibodies, induce the release of cytokines, and mediate binding to epithelial and endothelial cells
Virulence Factors:
Cell-Associated Components:
Peptidoglycan and Lipoteichoic Acid
Cell wall components function as virulence factors probably by stimulating cytokine release
Virulence Factor:
Cell-Associated Components:
Adhesins
Proteins of the cell surface promote attachement to host proteins, such as fibronectin. (Fibronectin-Binding Protein responsible for attachement to blood clots)
Besides binding to host matrix proteins, the adhesins are responsible for the formation of Biofilm
Virulence Factor:
Exoenzymes:
Coagulase
Enzyme binds to prothrombin to form a complex called Staphylothrombin.
Protease activity is activated in the complex, leads to conversion of fibrinogen to fibrin
Localized clotting protects bacterial cells from phygocytic and immune defenses
Virulence Factor:
Exoenzymes:
Lipase
Enzyme hydrolyzes lipid molecules that the host produces in response to infections.
Act as surfactants and disrupt bacterial cell membranes, especially when an abscess is formed
Virulence Factor:
Exoenzyme:
Hyaluronidase
Enzyme digest hyaluronic acid present in connective tissue and promotes spread of the infection