Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Micro Lab Practical #1 > Stains- Lab 4 > Flashcards

Stains- Lab 4 Flashcards

1
Q

What shape is a coccus?

A

Circle

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What does the gram stain, stain?

A

The cell NOT PG

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What technique does the gram stain use?

A

Gram stain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Name the steps for gram stains

A
  1. Differential stain
  2. Primary stain: crystal violet
  3. Counter stain: safranin red
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What color is a gram positive?

A

Purple

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What color is a gram negative?

A

Red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Name the gram positive organism tested in lab

A

Bacillus subtilis (rods)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Name the gram negative organism tested in lab

A

Escherichia coli (E. coli) (rods)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What does the endospores stain, stain?

A

Endospores (vs. vegetative cells)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Name the steps of the crystal violet method (endospores)

A
  1. Negative stain for endospores
    - crystal violet- purple vegetative cells
    - endospores - clear
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Name the steps for the Schaeffer-Fulton method (endospores)

A
  1. Special stain
    - endospores- green
    - Vegetative cells - red
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What was the organism tested in the endospore stain?

A

Bacillus subtilius

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What is stained in the acid-fast stain?

A

Mycolic acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What technique does the AF stain use?

A

Ziegler-Neelsen technique

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What methods does the endospore stain use to stain ?

A

Crystal violet method & Schaeffer- Fulton method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Name the steps in the Ziehl-Neelsen technique (AF)

A
  1. Differential stain
  2. Primary stain: Carbol fuchsin
  3. Counterstain: methylene blue
17
Q

What color is a AF?

18
Q

What color is a non-AF?

19
Q

Name the organisms tested in lab for AF stain

A
  1. Mycobacterium smegmatis (AF rods)

2. Staphylococcus epidermis (non-AF cocci)

20
Q

What does the capsule stain, stain?

21
Q

What method does the capsule stain use?

A

Capsule stain

22
Q

Name the steps of the capsule stain

A
  1. Special stain
  2. Negative stain for capsule
    - India ink: black background
    - capsules: clear
    - cells: red
23
Q

What does the flagella stain, stain?

24
Q

What technique does the flagella stain use?

A

Leifsons technique or flagella stain

25
Q

Name the steps of the flagella stain

A
  1. Special stain
    - Mordant adheres to flagella
    - rolling drop slide
26
Q

What was the organism used in the flagella stain lab

A

Proteus vulgaris

27
Q

What is the only stain that had mixed culture? (Rods& coccus)

A

Acid fast stain

28
Q

What shape is a rod?

Micro Lab Practical #1 (12 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions