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Micro Lab Practical #1 > Stains- Lab 4 > Flashcards

Stains- Lab 4 Flashcards

1
Q

What shape is a coccus?

A

Circle

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2
Q

What does the gram stain, stain?

A

The cell NOT PG

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3
Q

What technique does the gram stain use?

A

Gram stain

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4
Q

Name the steps for gram stains

A
  1. Differential stain
  2. Primary stain: crystal violet
  3. Counter stain: safranin red
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5
Q

What color is a gram positive?

A

Purple

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6
Q

What color is a gram negative?

A

Red

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7
Q

Name the gram positive organism tested in lab

A

Bacillus subtilis (rods)

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8
Q

Name the gram negative organism tested in lab

A

Escherichia coli (E. coli) (rods)

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9
Q

What does the endospores stain, stain?

A

Endospores (vs. vegetative cells)

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10
Q

Name the steps of the crystal violet method (endospores)

A
  1. Negative stain for endospores
    - crystal violet- purple vegetative cells
    - endospores - clear
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11
Q

Name the steps for the Schaeffer-Fulton method (endospores)

A
  1. Special stain
    - endospores- green
    - Vegetative cells - red
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12
Q

What was the organism tested in the endospore stain?

A

Bacillus subtilius

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13
Q

What is stained in the acid-fast stain?

A

Mycolic acid

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14
Q

What technique does the AF stain use?

A

Ziegler-Neelsen technique

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15
Q

What methods does the endospore stain use to stain ?

A

Crystal violet method & Schaeffer- Fulton method

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16
Q

Name the steps in the Ziehl-Neelsen technique (AF)

A
  1. Differential stain
  2. Primary stain: Carbol fuchsin
  3. Counterstain: methylene blue
17
Q

What color is a AF?

A

Pink

18
Q

What color is a non-AF?

A

Blue

19
Q

Name the organisms tested in lab for AF stain

A
  1. Mycobacterium smegmatis (AF rods)

2. Staphylococcus epidermis (non-AF cocci)

20
Q

What does the capsule stain, stain?

A

Capsule

21
Q

What method does the capsule stain use?

A

Capsule stain

22
Q

Name the steps of the capsule stain

A
  1. Special stain
  2. Negative stain for capsule
    - India ink: black background
    - capsules: clear
    - cells: red
23
Q

What does the flagella stain, stain?

A

Flagella

24
Q

What technique does the flagella stain use?

A

Leifsons technique or flagella stain

25
Q

Name the steps of the flagella stain

A
  1. Special stain
    - Mordant adheres to flagella
    - rolling drop slide
26
Q

What was the organism used in the flagella stain lab

A

Proteus vulgaris

27
Q

What is the only stain that had mixed culture? (Rods& coccus)

A

Acid fast stain

28
Q

What shape is a rod?

A

Rod

Micro Lab Practical #1 (12 decks)

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