Stains

Question 1

What are the 3 main stains that we use?

Answer 1

Haematoxylin and eosin (H&E)
Masson’s trichrome
Elastic van Gieson (EVG)

Question 2

What 2 colors does the H&E stain produce?

Answer 2

Purple

Pink

Question 3

What is the name given to a structure that turns purple when stained with H&E?

Answer 3

Basophilic

Question 4

What is the name given to a structure that turns pink when stained with H&E?

Answer 4

Eosinophilic

Question 5

What does the H&E stain stand for?

Answer 5

Haematoxylin and Eosin

Question 6

What structures turn purple when stained with H&E?

Answer 6

Nuclei

Ribosomes

Question 7

What structures turn pink when stained with H&E?

Answer 7

Muscle filaments
Collagen
Cytoplasm
Extracellular matrix

Question 8

What structure turns blue or green when stained with Masson’s trichrome?

Answer 8

Collagen

Question 9

What structures turn red when stained with Masson’s trichrome?

Answer 9

Muscle
Cytoplasm
Blood

Question 10

What structure turns black when stained with Masson’s trichrome?

Answer 10

Nuclei

Question 11

What structure turns red when stained with Elastin van Geison (EVG)?

Answer 11

Collagen

Question 12

What structure turns yellow when stained with Elastin van Geison (EVG)?

Answer 12

Muscle

Question 13

What structure turns black when stained with Elastin van Geison (EVG)?

Answer 13

Elastin